{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T04:56:48Z","timestamp":1791349008427,"version":"4.3.1"},"reference-count":52,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2016,5,25]],"date-time":"2016-05-25T00:00:00Z","timestamp":1464134400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Grant-in-Aid for Scientific Research","award":["21791448"],"award-info":[{"award-number":["21791448"]}]},{"name":"Grant-in-Aid for Scientific Research","award":["25861374"],"award-info":[{"award-number":["25861374"]}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Science, Sports and Culture of Japan","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European Journal of Pain"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Decreased Gamma\u2010aminobutyric acid (GABA)\u2010ergic phasic inhibitory transmission in the spinal cord is thought to be responsible for the development of neuropathic pain. However, the role of GABAergic tonic current in substantia gelatinosa (SG) neurons in neuropathic pain remains to be fully elucidated. In this study, we assessed GABAergic tonic currents of SG neurons in a sciatic nerve chronic constriction injury (CCI) mouse.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>Whole\u2010cell patch clamp recordings form lumbar spinal cord slices was performed to evaluate GABAergic currents. We also investigated the expression changes of GABA<jats:sub>A<\/jats:sub> receptor subunits which are considered to mediate tonic currents.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The percentage of SG neurons receiving GABAergic tonic currents decreased in CCI mice compared with Na\u00efve mice. No significant change was observed in the mean amplitude of GABAergic tonic currents. RT\u2010PCR and Western blot revealed that the expression of GABA<jats:sub>A<\/jats:sub> receptor \u03b4 subunits decreased following CCI.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>A reduction in the expression the \u03b4 subunit of the GABA<jats:sub>A<\/jats:sub> receptor and diminished GABAergic tonic current in SG neurons were observed after CCI in mice. GABAergic tonic current plays a key role in neuropathic pain. The GABA<jats:sub>A<\/jats:sub> receptor \u03b4 subunit may be a therapeutic target in neuropathic pain.<\/jats:p><\/jats:sec><jats:sec><jats:title>What does this study add?<\/jats:title><jats:p>In spinal SG neurons, GABAergic inhibitory transmission operates through both phasic and tonic currents, but physiological role is largely unknown. In this study, we report dysregulation of GABA<jats:sub>A<\/jats:sub> receptor \u03b4 subunit\u2010mediated tonic current in SG neurons may result in spinal disinhibition resulting in neuropathic pain in CCI mice.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ejp.891","type":"journal-article","created":{"date-parts":[[2016,5,25]],"date-time":"2016-05-25T09:29:40Z","timestamp":1464168580000},"page":"1678-1688","source":"Crossref","is-referenced-by-count":19,"title":["Reductions in tonic <scp>GABA<\/scp>ergic current in substantia gelatinosa neurons and <scp>GABA<sub>A<\/sub><\/scp> receptor \u03b4 subunit expression after chronic constriction injury of the sciatic nerve in mice"],"prefix":"10.1002","volume":"20","author":[{"given":"A.","family":"Iura","sequence":"first","affiliation":[{"name":"Department of Anesthesiology and Intensive Care Medicine Graduate School of Medicine Osaka University 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