{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:42:17Z","timestamp":1786977737874,"version":"build-2736575974"},"reference-count":58,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2016,6,5]],"date-time":"2016-06-05T00:00:00Z","timestamp":1465084800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BPD\/87537\/2012"],"award-info":[{"award-number":["SFRH\/BPD\/87537\/2012"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European Journal of Pain"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Amylin is a peptide from the calcitonin gene\u2010related peptides (\n                      <jats:styled-content style=\"fixed-case\">CGRP<\/jats:styled-content>\n                      ) family that is expressed by nociceptors. Amylin may modulate pain via a spinal action.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The effect of amylin's administration on the formalin test of acute and tonic pain was evaluated. Amylin's ability to modulate neuronal activity was analysed by c\u2010Fos expression at the spinal cord lumbar 4\u20135 region (L4\u20105) and brain.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Amylin subcutaneous administration 20\u00a0min prior, but not immediately before formalin, shortened the interphase and anticipated the beginning of the tonic pain phase. Amylin reduced the number of activated spinal cord neurons. Blockade of spinal amylin\u2010receptors by prior L4\u20105 intrathecal administration of an amylin\u2010receptor antagonist (\n                      <jats:styled-content style=\"fixed-case\">AC<\/jats:styled-content>\n                      187) attenuated these effects, whereas intrathecal\n                      <jats:styled-content style=\"fixed-case\">BIBN<\/jats:styled-content>\n                      4096 (\n                      <jats:styled-content style=\"fixed-case\">CGRP<\/jats:styled-content>\n                      \u2010receptor antagonist) did not, proving that part of amylin's effects were spinally mediated via amylin\u2010receptors. The locus coeruleus and other areas involved in descending modulation and affective responses to pain showed an increased number of activated neurons upon amylin subcutaneous administration, suggesting a role for supraspinal areas in some observed effects. L4\u20105 intrathecal injection of amylin or\n                      <jats:styled-content style=\"fixed-case\">AC<\/jats:styled-content>\n                      187 showed that both ligands attenuated tonic pain, but blockade of the amylin\u2010receptor action by\n                      <jats:styled-content style=\"fixed-case\">AC<\/jats:styled-content>\n                      187 decreased further the number of paw jerks in this period.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Overall, data suggested that amylin modulates pain with an inflammatory component and the autoanalgesic\/inhibitory mechanisms occurring in the interphase of the formalin test. Amylin might have affected the nociceptive system at different levels (spinal cord and brain), explaining the different effects observed according to the time of amylin injection.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>What does this study add?<\/jats:title>\n                    <jats:p>\n                      <jats:list list-type=\"bullet\">\n                        <jats:list-item>\n                          <jats:p>Amylin modulated formalin interphase and tonic pain behaviours probably by targeting spinal neurons and affecting supraspinal areas involved in affective and modulatory components of pain.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Activation of spinal amylin\u2010receptors may contribute to the initiation of inflammatory pain mechanisms.<\/jats:p>\n                        <\/jats:list-item>\n                      <\/jats:list>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ejp.898","type":"journal-article","created":{"date-parts":[[2016,6,5]],"date-time":"2016-06-05T20:56:44Z","timestamp":1465160204000},"page":"1741-1752","source":"Crossref","is-referenced-by-count":13,"title":["Amylin modulates the formalin\u2010induced tonic pain behaviours in rats"],"prefix":"10.1002","volume":"20","author":[{"given":"C.S.","family":"Potes","sequence":"first","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade Universidade do Porto Porto Portugal"},{"name":"IBMC\u2010Instituto de Biologia Molecular e Celular Universidade do Porto Porto Portugal"},{"name":"Departamento de Biologia Experimental Faculdade de Medicina Universidade do Porto Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.C.","family":"Pestana","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Experimental Faculdade de Medicina Universidade do Porto Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Pontes","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Experimental Faculdade de Medicina Universidade do Porto Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.S.","family":"Caramelo","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Experimental Faculdade de Medicina Universidade do Porto Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.L.","family":"Neto","sequence":"additional","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade Universidade do Porto Porto Portugal"},{"name":"IBMC\u2010Instituto de Biologia Molecular e Celular Universidade do Porto Porto Portugal"},{"name":"Departamento de Biologia Experimental Faculdade de Medicina Universidade do Porto Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,6,5]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3959(96)03285-X"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1460-9568.1999.00411.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2005.11.014"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2011.01717.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(95)02197-Q"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(99)00478-9"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2004.10.012"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M413786200"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-0115(95)00009-Z"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2013.06.021"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4179(90)90055-4"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.56.1.235"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.20669"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3959(87)90023-6"},{"key":"e_1_2_7_16_1","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/S0022-3565(25)12039-9","article-title":"Intrathecal baclofen and muscimol, but not midazolam, are antinociceptive using the rat\u2010formalin model","volume":"275","author":"Dirig D.M.","year":"1995","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0703110"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3959(77)90130-0"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0091-3057(93)90558-B"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-328X(98)00269-1"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3959(95)00141-7"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1090-3801(97)90067-7"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0361-9230(97)00277-3"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2006.01.036"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2009.11.063"},{"key":"e_1_2_7_26_1","doi-asserted-by":"crossref","first-page":"928","DOI":"10.1016\/S0022-3565(24)36830-2","article-title":"Role of spinal gamma\u2010aminobutyric acidA receptors in formalin\u2010induced nociception in the rat","volume":"282","author":"Kaneko M.","year":"1997","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_7_27_1","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1016\/S0022-3565(24)35347-9","article-title":"Intrathecally administered gabapentin inhibits formalin\u2010evoked nociception and the expression of Fos\u2010like immunoreactivity in the spinal cord of the rat","volume":"292","author":"Kaneko M.","year":"2000","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0050361"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1213\/ane.0b013e318163f602"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-200401190-00014"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(97)00662-3"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389450053174596"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0196-9781(97)00292-1"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresbull.2008.09.005"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3959(98)00194-8"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(96)12552-0"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2009.04.032"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2011.01.012"},{"key":"e_1_2_7_39_1","first-page":"A939","article-title":"The formalin test: Time course and location changes of c\u2010Fos mRNA and FOS protein expression","volume":"99","author":"Mizobuchi S.","year":"2003","journal-title":"Anesthesiology"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.140.6.6930"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.15-11-07625.1995"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-328X(97)00178-2"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.3758\/BF03200814"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2012.08273.x"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1177\/1073858403253660"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2006.07.089"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3959(95)00121-2"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00213.2004"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.20384"},{"key":"e_1_2_7_50_1","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1016\/S0022-3565(25)22436-3","article-title":"Autoradiographic distribution and receptor binding profile of [125I]Bolton Hunter\u2010rat amylin binding sites in the rat brain","volume":"270","author":"Rossum D.","year":"1994","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(94)90388-3"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3959(89)90222-4"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(99)02286-6"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00339.2004"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(00)01389-6"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3959(92)90003-T"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.10478"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-0115(94)90131-7"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3959(83)90201-4"}],"container-title":["European Journal of Pain"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fejp.898","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ejp.898","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T16:44:40Z","timestamp":1748969080000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ejp.898"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,6,5]]},"references-count":58,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2016,11]]}},"alternative-id":["10.1002\/ejp.898"],"URL":"https:\/\/doi.org\/10.1002\/ejp.898","archive":["Portico"],"relation":{},"ISSN":["1090-3801","1532-2149"],"issn-type":[{"value":"1090-3801","type":"print"},{"value":"1532-2149","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,6,5]]}}}