{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:41:47Z","timestamp":1760575307704,"version":"build-2065373602"},"reference-count":20,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,4,13]],"date-time":"2005-04-13T00:00:00Z","timestamp":1113350400000},"content-version":"vor","delay-in-days":6312,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[1988,1]]},"abstract":"Abstract<\/jats:title>In the course of a population study of \u03b11<\/jats:sub>\u2010antitrypsin polymorphism by separator isoelectric focusing, a variant phenotype having a somewhat narrower spacing than PI M1M3 was observed in a Japanese blood donor. Family studies by hybrid isoelectric focusing in a carrier ampholyte\u2010supplemented immobilized pH gradient from 4.35\u20134.65 revealed that the products of the responsible gene, PI<\/jats:italic>*Mtoyoura<\/jats:italic>, were extremely close but slightly cathodal to those of PI<\/jats:italic>*M1.<\/jats:italic> The difference in isoelectric point between them corresponded to the resolving limit of isoelectric focusing. For this reason, although the propositus' father was deduced to have the genotype PI<\/jats:italic>*M1<\/jats:italic>\/PI<\/jats:italic>*Mtoyoura<\/jats:italic>, the products of these two genes failed to form a double band pattern. Thus, the gene frequency for PI<\/jats:italic>*Mtoyoura<\/jats:italic> was unknown. These findings, however, indicate that a further microheterogeneity in the PI M<\/jats:bold> subtype exists at least in the Japanese.<\/jats:p>","DOI":"10.1002\/elps.1150090311","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T08:58:07Z","timestamp":1117270687000},"page":"151-153","source":"Crossref","is-referenced-by-count":8,"title":["PI Mtoyoura: A new PI M subtype found by separator and hybrid isoelectric focusing"],"prefix":"10.1002","volume":"9","author":[{"given":"Isao","family":"Yuasa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuyuki","family":"Suenaga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuo","family":"Umetsu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Osamu","family":"Inagaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Ikebuchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keiichi","family":"Ito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kichiro","family":"Okada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,4,13]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1159\/000152959"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00329137"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00295662"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150070505"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(01)86910-7"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00273002"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150060704"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150070509"},{"volume-title":"LKB Application Note 346","author":"Altland K.","key":"e_1_2_1_10_2"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00295372"},{"key":"e_1_2_1_12_2","first-page":"863","volume":"34","author":"Weidinger S.","year":"1986","journal-title":"Prot. 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