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To resolve the many components in transferrin preparations, the new high performance capillary technique was employed for isoelectric focusing. Iron\u2010free transferrin and transferrin samples of known iron content were examined. The above method gives an exceptionally rapid analysis (within 15\u201325 min) of small amounts of samples (less than 1 \u03bcg protein) and as good as or better resolution than other isoelectric focusing techniques previously used for transferrin analysis. By monitoring the focused protein zones at both 280 and 460 nm the molecular forms of transferrin (iron\u2010free, monoferric and differic complexes) can easily be identified. Both steps of isoelectric focusing in capillaries (<jats:italic>i. e.<\/jats:italic>, prefocusing and mobilization) can be used for analysis. We observed that chelating agents (<jats:italic>e. g.<\/jats:italic>, carrier ampholytes, nitrilotriacetate) may release iron from micro\u2010syringes having metal pistons causing the formation of iron\u2010transferrin complexes.<\/jats:p>","DOI":"10.1002\/elps.1150100107","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T09:01:58Z","timestamp":1117270918000},"page":"23-29","source":"Crossref","is-referenced-by-count":163,"title":["Fast and high resolution analysis of human serum transferrin by high performance isoelectric focusing in capillaries"],"prefix":"10.1002","volume":"10","author":[{"given":"Ferenc","family":"Kil\u00e1r","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stellan","family":"Hjert\u00e9n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,4,13]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(83)90250-4"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(83)90244-9"},{"key":"e_1_2_1_4_2","first-page":"1","volume":"34","author":"van Noort W. 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