{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T01:14:04Z","timestamp":1760577244425,"version":"build-2065373602"},"reference-count":23,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2005,4,13]],"date-time":"2005-04-13T00:00:00Z","timestamp":1113350400000},"content-version":"vor","delay-in-days":5946,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[1989,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>By means of two\u2010dimensional lectin affinity electrophoresis of human \u03b1\u2010fetoprotein (AFP) from different sources, AFP bands separated with erythroagglutinating phytohemagglutinin (E\u2010PHA) were further characterized with other lectins of known oligosaccharide specificities. The results with a cord serum AFP revealed that not only AFP\u2010P2 (E\u2010PHA\u2010nonreactive) but also AFP\u2010P4 and P5 (E\u2010PHA\u2010reactive) had affinities for Concanavalin A (Con A) and <jats:italic>Allomyrina dichotoma<\/jats:italic> lectin (allo A), indicating that the cord serum AFP has nonbisected biantennary complex\u2010type oligosaccharides with the terminal galactose on Man \u03b11 \u2192 6 residue sialylated at the C\u20106, but not C\u20103, position. On the other hand, the results with a hepatoblastoma (HUH\u20106 C1\u20135 cell line) AFP showed that not only AFP\u2010P5 but also AFP\u2010P1 (E\u2010PHA\u2010nonreactive) and P3 (E\u2010PHA\u2010less reactive) had Con A\u2010nonreactive AFP and that AFP\u2010P1 had AFP\u2010A1 (allo A\u2010nonreactive) and AFP\u2010A2 (allo A\u2010less reactive), and AFP\u2010P3 and P4 had AFP\u2010A1s (allo A\u2010nonreactive), as main components, in addition to the spots of cord serum AFP. Most of the E\u2010PHA\u2010dependent bands of AFP were further subdivided with <jats:italic>Lens culinaris<\/jats:italic> agglutinin (LCA\u2010A) into LCA\u2010A\u2010reactive, weakly reactive and nonreactive spots. Similar results were obtained with AFP preparations from hepatocullular carcinomas and other malignancies, indicating that the bisected bi\u2010(or tri\u2010and tetra\u2010) antennary sugar chains with the exposed terminal galactose of the Man \u03b1 1 \u2192 6 arm as well as those with the C\u20103 sialylated galactose residues could be expressed in AFP upon malignant transformation. The two\u2010dimensional lectin affinity electrophoresis thus proves useful for further characterization of lectin\u2010dependent AFP bands.<\/jats:p>","DOI":"10.1002\/elps.1150101205","type":"journal-article","created":{"date-parts":[[2005,5,27]],"date-time":"2005-05-27T18:44:41Z","timestamp":1117219481000},"page":"825-829","source":"Crossref","is-referenced-by-count":23,"title":["Two\u2010dimensional lectin affinity electrophoresis of \u03b1\u2010fetoprotein: Characterization of erythroagglutinating phytohemagglutinin\u2010dependent microheterogeneity forms"],"prefix":"10.1002","volume":"10","author":[{"given":"Kazuhisa","family":"Taketa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eriko","family":"Ichikawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiro","family":"Sato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroko","family":"Taga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidematsu","family":"Hirai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,4,13]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"4276","volume":"40","author":"Yoshima H.","year":"1980","journal-title":"Cancer Res."},{"key":"e_1_2_1_3_2","first-page":"4691","volume":"43","author":"Yamashita K.","year":"1983","journal-title":"Cancer Res."},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1981.tb00179.x"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.2957\/kanzo.22.1559"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1975.tb03820.x"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19880215)61:4<769::AID-CNCR2820610422>3.0.CO;2-M"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150100805"},{"key":"e_1_2_1_9_2","first-page":"519","volume":"6","author":"Taketa K.","year":"1985","journal-title":"Tumour Biol."},{"key":"e_1_2_1_10_2","first-page":"533","volume":"6","author":"Taketa K.","year":"1985","journal-title":"Tumour Biol."},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3835(86)90155-2"},{"key":"e_1_2_1_12_2","first-page":"627","volume-title":"Electrophoresis '83","author":"Takeo K.","year":"1984"},{"key":"e_1_2_1_13_2","first-page":"137","volume-title":"Electrophoresis '84","author":"Taketa K.","year":"1984"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150061005"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150080908"},{"key":"e_1_2_1_16_2","first-page":"133","volume":"2","author":"Taketa K.","year":"1987","journal-title":"J. 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