{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T01:00:29Z","timestamp":1760576429525,"version":"build-2065373602"},"reference-count":17,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,4,13]],"date-time":"2005-04-13T00:00:00Z","timestamp":1113350400000},"content-version":"vor","delay-in-days":5581,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[1990,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In order to detect histamine receptors on the surface of human peripheral blood monouclear cells, the cells were incubated in the presence of radiolabelled histamine and then the bifunctional crosslinker disuccimidyl suberate was added in various concentrations. They were then solubilized with sodium dodecyl sulphate, boiled, reduced and the lysate separated by sodium dodecyl sulfate\u2010polyacrylamide gel electrophoresis. Both <jats:sup>3<\/jats:sup>H and <jats:sup>125<\/jats:sup>I\u2010radiolabelled ligands bound to a 16 kDa band, to be defined although a much clearer and obviously unequivocal signal was obtained with <jats:sup>3<\/jats:sup>H\u2010labelled histamine. This molecule migrated with the same mass on sodium dodecyl sulfate\u2010polyacrylamide gel electrophoresis as a 16 kDa subunit which had been purified on a histamine affinity column from Triton X\u2010100 solubilized mononuclear cells, indicating it to be the ligand\u2010binding subunit for the histamine receptor on these cells. For <jats:sup>3<\/jats:sup>H, fluorography with Entensify<jats:sup>TM<\/jats:sup> was required to obtain an autoradiographic signal. Although <jats:sup>3<\/jats:sup>H took much longer to give a signal than <jats:sup>125<\/jats:sup>I, the considerable background, artefacts and heavy lane trailing seen with [<jats:sup>125<\/jats:sup>I] histamine were completely abrogated when [<jats:sup>3<\/jats:sup>H]histamine was used. In addition, the distinction between specific and nonspecific binding was more clearly seen using [<jats:sup>3<\/jats:sup>H]histamine. The modifications reported here which improve signal detection for <jats:sup>3<\/jats:sup>H should encourage the use of tritiated ligands in radioreceptor crosslinking, particularly those of low molecular weight which might otherwise undergo steric modification due to iodination, this having the potential for interfering with receptor ligand binding.<\/jats:p>","DOI":"10.1002\/elps.1150110611","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T09:17:00Z","timestamp":1117271820000},"page":"495-499","source":"Crossref","is-referenced-by-count":0,"title":["Improved detection of the histamine receptor on human peripheral blood mononuclear cells by crosslinking using tritiated as compared with radioiodinated histamine"],"prefix":"10.1002","volume":"11","author":[{"given":"Robert S.","family":"Warlow","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan","family":"Dempsey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raeleen","family":"Carroll","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven","family":"Gibson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claude C. 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