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The glycoprotein was recovered by electroelution from a polyacrylamide gel strip and used for the production of monoclonal antibodies. Four of the five antibodies obtained bound specifically to the protein moiety of the contact site A glycoprotein. The specificities of the antibodies were in striking contrast to those of antibodies raised against the contact site A glycoprotein purified by Triton X\u2010114 phase separation and DEAE chromatography. The majority of the latter antibodies recognized the carbohydrate moiety of the contact site A glycoprotein and cross\u2010reacted heavily with other membrane glycoproteins.<\/jats:p>","DOI":"10.1002\/elps.1150111015","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T09:11:12Z","timestamp":1117271472000},"page":"856-860","source":"Crossref","is-referenced-by-count":2,"title":["Recovery of the contact site A glycoprotein of <i>Dictyostelium discoideum<\/i> from sodium dodecyl sulfatepolyacrylamide gel and characteristics of monoclonal antibodies against the recovered protein"],"prefix":"10.1002","volume":"11","author":[{"given":"Hiroshi","family":"Ochiai","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimiaki","family":"Jin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noriaki","family":"Kakihara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamao","family":"Saito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masashi","family":"Fukuzawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,4,13]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.56.3.647"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/274445a0"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1979.tb13271.x"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/jsscb.380170302"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1515\/bchm2.1982.363.2.771"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1982.tb01286.x"},{"key":"e_1_2_1_8_2","first-page":"1604","volume":"256","author":"Hohmann H \u2010P.","year":"1985","journal-title":"J. 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