{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:54:18Z","timestamp":1760576058841,"version":"build-2065373602"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,4,13]],"date-time":"2005-04-13T00:00:00Z","timestamp":1113350400000},"content-version":"vor","delay-in-days":4485,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[1993,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In this paper, we summarize our five\u2010year observation of two\u2010dimensional polyacrylamide gel electrophoretic (2\u2010D PAGE) analysis of immunoglobulin (Ig) light (L) chain patterns on serum\/plasma and\/or purified human Ig, and compare this technique with agarose electrophoresis and\/or immunofixation examination. Polyclonal Ig L chains were seen as large \u201cfuzzy\u201d areas with several zones of high density. The majority (71%) of the monoclonal Ig L chains of monoclonal gammopathy detected by conventional electrophoresis appeared as a single large and well\u2010defined spot on 2\u2010D PAGE analysis, with the remaining appearing as multiple spots. The presence of oligoclonal Ig, reflected by multiple spots in 2\u2010D PAGE, and several bands in immunofixation, was observed in 5 of 26 patients after allogeneic bone marrow transplantation and in 5 patients with tumors. In the majority (77%) of hypergamma\u2010globulinemia, L chains appeared as a wide spread of small and well\u2010defined spots in 2\u2010D PAGE analysis. This pattern suggested oligoclonal Ig\u2010secreting B cell clone expansion, and corresponding abnormalities were not detected with immunofixation. 2\u2010D PAGE analysis also detected oligoclonal Ig expansions whereas conventional electrophoretic examination was normal in 10 additional patients after bone marrow transplantation, and in 6 of 10 immunocompetent patients with acute severe infections. Analysis of the Ig L chain pattern of a severe combined immune\u2010deficient mouse populated with human peripheral blood leukocytes confirmed the skewed human Ig production in the model. In summary, 2\u2010D PAGE appears to be a sensitive tool for the analysis of Ig diversity in various clinical situations.<\/jats:p>","DOI":"10.1002\/elps.11501401210","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T09:32:37Z","timestamp":1117272757000},"page":"1366-1371","source":"Crossref","is-referenced-by-count":12,"title":["Two\u2010dimensional electrophoresis as an aid in the analysis of the clonality of immunoglobulins"],"prefix":"10.1002","volume":"14","author":[{"given":"Jean\u2010Daniel","family":"Tissot","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philippe","family":"Schneider","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"Hohlfeld","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fran\u00e7ois","family":"Spertini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denis F.","family":"Hochstrasser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michel A.","family":"Duchosal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,4,13]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_1_2_2","DOI":"10.1016\/0092-8674(91)90032-T"},{"key":"e_1_2_1_3_2","doi-asserted-by":"crossref","first-page":"2181","DOI":"10.1182\/blood.V79.9.2181.2181","volume":"79","author":"Cogn\u00e9 M.","year":"1992","journal-title":"Blood"},{"doi-asserted-by":"publisher","key":"e_1_2_1_4_2","DOI":"10.1016\/S0065-2776(08)60876-7"},{"doi-asserted-by":"publisher","key":"e_1_2_1_5_2","DOI":"10.1016\/S0889-8588(18)30348-4"},{"issue":"2","key":"e_1_2_1_6_2","first-page":"24","volume":"29","author":"Greipp P. 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