{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T04:50:33Z","timestamp":1761540633887,"version":"build-2065373602"},"reference-count":15,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,4,13]],"date-time":"2005-04-13T00:00:00Z","timestamp":1113350400000},"content-version":"vor","delay-in-days":4485,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[1993,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To study the clonal events occurring during ontogeny of the humoral immune system, we evaluated plasma immunoglobulin (Ig) production in term newborns and young children by high\u2010resolution two\u2010dimensional gel electrophoresis. The clonality pattern of Ig light (L) chains from healthy newborns (<jats:italic>n<\/jats:italic> = 19) was similar to that observed on protein maps of their mothers or of normal adults (<jats:italic>n<\/jats:italic> &gt; 100), that is, rare distinguishable small spots in a cloud\u2010like large band of indiscrete Ig L chain spots (polyclonal pattern; maternal Igs). Analysis of plasma samples obtained from infants between 1 month and 5 years of age (<jats:italic>n<\/jats:italic> = 55) revealed discrete but evident alterations of the clonality of Ig production. Between the 2nd and 4th months of life, transient attenuation of the \u201cpolyclonal background\u201d was observed in association with the appearance of an increasing number of well\u2010resolved Ig L chain spots (corresponding to plasma Ig concentrations between 0.5 and 1 g\/L per spot). This \u201crestricted\u201d clonal pattern was progressivly less apparent on protein maps of infants older than 2 year and evolved towards a \u201cnormal\u201d adult polyclonal pattern at the age of 5. These results suggest that the development of the B\u2010cell clones is heterogeneous, either through limited outgrowth of precursor cells or through selective antigenic pressures.<\/jats:p>","DOI":"10.1002\/elps.1150140142","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T09:32:37Z","timestamp":1117272757000},"page":"245-247","source":"Crossref","is-referenced-by-count":14,"title":["Clonal imbalances of plasma\/serum immunogloblin production in infants"],"prefix":"10.1002","volume":"14","author":[{"given":"Jean\u2010Daniel","family":"Tissot","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"Hohlfeld","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denis F.","family":"Hochstrasser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean\u2010Fran\u00e7ois","family":"Tolsa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9","family":"Calme","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philippe","family":"Schneider","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,4,13]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0046-8177(84)80051-9"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1990.tb04362.x"},{"key":"e_1_2_1_4_2","first-page":"7","volume":"2","author":"Tissot J. 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