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Optical isomers of vanilmandelic acid, warfarin, ibuprofen, ketoprofen, flurbiprofen, and folinic acid were successfully resolved within 20 min with 25 \u03bc<jats:sc>M<\/jats:sc> avidin in a linear polyacrylamide\u2010coated capillary under weakly acidic conditions. The effects of pH, the concentration of avidin, addition of an organic solvent such as ethanol and 2\u2010propanol, and temperature on enantioselectivity were investigated. The choice of pH and the organic solvent additive was critical to improve the separation. Some general considerations about affinity EKC are also described, based on a simple one\u2010site interaction model.<\/jats:p>","DOI":"10.1002\/elps.11501501120","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T10:12:25Z","timestamp":1117275145000},"page":"848-853","source":"Crossref","is-referenced-by-count":76,"title":["Separation of enantiomers by affinity electrokinetic chromatography using avidin"],"prefix":"10.1002","volume":"15","author":[{"given":"Yoshihide","family":"Tanaka","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norio","family":"Matsubara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigeru","family":"Terave","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,4,13]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(92)80145-K"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02290245"},{"key":"e_1_2_1_4_2","first-page":"617","volume-title":"Capillary Electrophoresis Technology","author":"Otsuka K.","year":"1993"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(94)80392-7"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(01)84309-0"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(01)89318-3"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/jhrc.1240130814"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00192a043"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(91)80071-N"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-9936(89)85022-8"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(93)83435-U"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00047a026"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(93)83121-8"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(93)83437-W"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(93)83436-V"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(94)80381-1"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(92)85366-2"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00068a028"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00063a014"}],"container-title":["ELECTROPHORESIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Felps.11501501120","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.11501501120","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T15:43:11Z","timestamp":1760542991000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/elps.11501501120"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,1]]},"references-count":19,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1994,1]]}},"alternative-id":["10.1002\/elps.11501501120"],"URL":"https:\/\/doi.org\/10.1002\/elps.11501501120","archive":["Portico"],"relation":{},"ISSN":["0173-0835","1522-2683"],"issn-type":[{"type":"print","value":"0173-0835"},{"type":"electronic","value":"1522-2683"}],"subject":[],"published":{"date-parts":[[1994,1]]}}}