{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:53:54Z","timestamp":1760576034162,"version":"build-2065373602"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,4,13]],"date-time":"2005-04-13T00:00:00Z","timestamp":1113350400000},"content-version":"vor","delay-in-days":4120,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[1994,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Two\u2010dimensional polyacrylamide gel electrophoresis (2\u2010D PAGE) was used to identify cellular proteins in T and B acute lymphoblastic leukemia (ALL) cell lines. Five lines, REH and BALL\u20101 of B\u2010cell lineage, CCRF\u2010CEM and HPB\u2010ALL of T\u2010cell lineage, and a normal Epstein\u2010Barr virus (EBV)\u2010transformed line of B\u2010origin (SKLN1) were studied. The lines were immunophenotyped using flow cytometry and lineage associated monoclonal antibodies. Whole cell lysates of the cell lines were subjected to 2\u2010D PAGE analyses. 2\u2010D gels were analyzed with an image scanning computer and the qualitative as well as quantitative differences of the protein patterns were studied. Despite the great similarities in the patterns of the B\u2010 and T\u2010gels, three proteins were unique to B\u2010cell lines, while eight were unique to T\u2010cell lines. Using cell lines is the first step toward identifying potential markers in ALL and can provide important information regarding the human ALL databases. Whether these proteins are definite markers for B\u2010 or T\u2010ALL or are unique to the cell lines studied needs further exploration.<\/jats:p>","DOI":"10.1002\/elps.11501501184","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T10:12:25Z","timestamp":1117275145000},"page":"1218-1224","source":"Crossref","is-referenced-by-count":3,"title":["Protein study of T and B acute lymphoblastic leukemia cell lines"],"prefix":"10.1002","volume":"15","author":[{"given":"Ramzi M.","family":"Mohammad","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kerry","family":"Vistisen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayad","family":"Al\u2010Katib","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,4,13]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1093\/clinchem\/28.4.828","volume":"28","author":"Lester E. 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