{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:53:54Z","timestamp":1760576034335,"version":"build-2065373602"},"reference-count":17,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,4,13]],"date-time":"2005-04-13T00:00:00Z","timestamp":1113350400000},"content-version":"vor","delay-in-days":4120,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[1994,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Capillary electrophoresis (CE) conditions have been developed for the separation of hydrophobic polypeptides, such as fatty acid\u2010acylated peptides, and their subsequent structural identification by <jats:sup>252<\/jats:sup>Cf plasma desorption (PDMS) and electrospray mass spectrometry (ESMS). Salt\u2010 and detergent\u2010free aqueous acetic acid buffers containing up to 20% 2\u2010propanol or 25% acetonitrile were employed for CE separations of hydrophobic peptides with (i) untreated, and (ii) 3\u2010aminopropyltrimethoxysilane\u2010derivatized fused silica capillaries. For both capillary types, electroosmotic flow rates suitable for sample isolation and transfer were determined, and CE separations of polypeptide mixtures were compared for aqueous buffers containing 2\u2010propanol or acetonitrile. For the mass spectrometric identification of CE\u2010separated peptides, a sheath flow sample isolation method was developed for subsequent transfer to PDMS. This procedure enabled the efficient isolation of peptide fractions for PDMS analysis, or alternative microanalytical techniques.<jats:fn><jats:p>Presented in part at the International Electrophoresis Forum, Munich, October 26\u201328, 1992.<\/jats:p><\/jats:fn>\n<\/jats:p>","DOI":"10.1002\/elps.1150150139","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T10:12:25Z","timestamp":1117275145000},"page":"228-233","source":"Crossref","is-referenced-by-count":22,"title":["Capillary electrophoresis combined with <sup>252<\/sup>Cf plasma desorption and electrospray mass spectrometry for the structural characterization of hydrophobic polypeptides using organic solvents"],"prefix":"10.1002","volume":"15","author":[{"given":"Wolfgang","family":"Weinmann","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carol E.","family":"Parker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kerstin","family":"Baumeister","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Maier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth B.","family":"Tomer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Przybylski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,4,13]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)35957-4"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4157(89)90013-0"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.104.6.1449"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00463a001"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(89)90417-0"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4889(89)90200-0"},{"volume-title":"Biological Mass Spectrometry","year":"1990","author":"Burlingame A. 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