{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T15:10:52Z","timestamp":1770822652608,"version":"3.50.1"},"reference-count":14,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,4,14]],"date-time":"2005-04-14T00:00:00Z","timestamp":1113436800000},"content-version":"vor","delay-in-days":3756,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[1995,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To analyze the transition of an autoimmune disease into a mucosa\u2010associated lymphoid tissue\u2010non\u2010Hodgkin's lymphoma (MALT\u2010NHL), we investigated a total of 27 cases of clinically diagnosed autoimmune thyroiditis with lymphoid hyperplasia. Three cases of thyroid hyperplasia served as controls. Monoclonal B cells were detected by studying rearrangement patterns of the hypervariable CDR III regions within the immunoglobulin heavy chain gene locus and the T\u2010cell receptor \u03b3 chain gene (TCRG). We used a seminested polymerase chain reaction (PCR) to demonstrate immunoglobulin rearrangements and a multiplex PCR for TCRG rearrangements. The PCR products were analyzed by temperature gradient gel electrophoresis to expand mixtures of homo\u2010 and hetero\u2010duplices within heterogeneous populations of B cells. With this approach we found monoclonality in 14 of the 27 cases of Hashimoto's disease. In a reinvestigation we discovered additional histological and immunohistochemical features of MALT\u2010NHL in 17 cases. The 14 cases of thyroiditis with clonally expanded B cells clearly demonstrate the transition from autoimmune disease to non\u2010Hodgkin's lymphoma.<\/jats:p>","DOI":"10.1002\/elps.11501601118","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T10:29:35Z","timestamp":1117276175000},"page":"729-732","source":"Crossref","is-referenced-by-count":4,"title":["Temperature gradient gel electrophoresis for analysis of clonal evolution in non\u2010Hodgkin's lymphoma of the thyroid"],"prefix":"10.1002","volume":"16","author":[{"given":"Markus","family":"Tiemann","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin A. O. 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