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The chiral resolution was strongly influenced by several important parameters: surfactant concentration and addition of organic modifier to the background electrolyte (BGE). An optimized electrophoretic system was used, consisting of the following conditions: 25 m<jats:sc>M<\/jats:sc> phosphate buffer, pH 7.8, 50 m<jats:sc>M<\/jats:sc> sodium dodecyl sulfate, 20 m<jats:sc>M<\/jats:sc> \u03b3\u2010CD, 7.4% <jats:sc>V<\/jats:sc>\/<jats:sc>V<\/jats:sc> 2\u2010propanol as BGE; the applied voltage, 18 kV, corresponded to 37 \u03bcA at a constant temperature of 25\u00b0C. This electrophoretic method was applied for monitoring the chry\u2010<jats:italic>trans<\/jats:italic>\u20101,2\u2010diOH enantiomer formation in a real sample, obtained from <jats:italic>in vitro<\/jats:italic> metabolic activation of chrysene by phenobarbital\u2010\u00df\u2010naphthoflavone\u2010induced rat microsomes. The (+) and (\u2010) enantiomers were identified by the racemate and the single enantiomer standard addition method and by spectra comparison with the synthetic compound. Under the experimental conditions used for chrysene activation, the (+) optical isomer was the prevailing form. The CD\u2010MEKC system showed high reproducibility and selectivity, allowing a fast and interference\u2010free analysis even of the <jats:italic>in vitro<\/jats:italic> metabolic sample extract, without any pretreatment.<\/jats:p>","DOI":"10.1002\/elps.11501601128","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T10:29:35Z","timestamp":1117276175000},"page":"784-788","source":"Crossref","is-referenced-by-count":7,"title":["Cyclodextrin modified micellar electrokinetic chromatography for the chiral direct resolution of (+), (\u2212)\u2010<i>trans<\/i>\u20101,2\u2010dihydrodiol metabolite of chrysene <i>in vitro<\/i> activated by rat liver microsome S9 fraction"],"prefix":"10.1002","volume":"16","author":[{"given":"Claudia","family":"Desiderio","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salvatore","family":"Fanali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Massimo","family":"Sinibaldi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiara","family":"Polcaro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,4,14]]},"reference":[{"volume-title":"Polycyclic Hydrocarbons and Cancer","year":"1978","author":"Gelboin H. 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