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Twenty\u2010five proteins were identified either by immunoblotting using monoclonal antibodies (MAbs) or by <jats:italic>N<\/jats:italic>\u2010terminal microsequencing. The protein spots identified by MAbs were the 89 kDa outer membrane protein, DnaK, bacterioferritin, CP24, and BP26. Some spots were identified by <jats:italic>N<\/jats:italic>\u2010terminal microsequencing as proteins whose sequences had been reported previously from <jats:italic>Brucella<\/jats:italic>, such as three heat\u2010shock proteins, namely DnaK, GroEL and GroES; bacterioferritin; Cu\u2010Zn superoxide dismutase; and the 50S ribosomal protein L7\/L12. Other proteins had amino acid sequences homologous with those of various proteins from other bacteria found in protein databases: ClpP; the 10K\u2010S protein; the ORFU phosphoprotein; succinyl\u2010CoA synthetase alpha subunit; an inorganic pyrophosphatase; the Fe and\/or Mn superoxide dismutase; the nucleoside diphosphate kinase, an amino acid ABC type transporter, and an electron transfer flavoprotein small subunit. Seven proteins were identified with <jats:italic>N<\/jats:italic>\u2010terminal sequences not yet reported in databases. The 2\u2010D map established in this study will be the basis for comparative studies of protein expression in <jats:italic>Brucella<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/elps.1150180128","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T11:29:59Z","timestamp":1117279799000},"page":"156-162","source":"Crossref","is-referenced-by-count":38,"title":["Mapping and identification of <i>Brucella melitensis<\/i> proteins by two\u2010dimensional electrophoresis and microsequencing"],"prefix":"10.1002","volume":"18","author":[{"given":"Ana P.","family":"Teixeira\u2010Gomes","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Axel","family":"Cloeckaert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guy","family":"B\u00e9zard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G\u00e9rard","family":"Dubray","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michel S.","family":"Zygmunt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,4,14]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"383","volume-title":"Animal Brucellosis","author":"Alton G. 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