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Initial investigations led to discoveries of synthetic and degradative pathways, and brief forays into functional determination of the \u201cglyco\u201d portion on the protein activity in glycoproteins. Only recently has it come to our understanding that variations from the \u201cnormal\u201d glycosylation patterns might be indicative of pathological states. The presence of certain transferrin (Tf) glycoforms in human serum has been shown to correlate with certain clinical syndromes. Hence, the ability to separate and quantitatively measure the various forms of human Tf has become increasingly important. It this study, we demonstrate that a simple method utilizing a DB\u201017\u2010coated capillary to slow endoosmotic flow and a sieving buffer containing hydroxyethyl cellulose allows for the resolution of sialoforms of transferrin. An analysis time of less than eight minutes allows for baseline resolution of the lower sialoforms of Tf, presenting a simple, rapid test for carbohydrate\u2010deficient transferrin (CDT). We demonstrate the utility of this methodology for the facile diagnosis of carbohydrate\u2010deficient glycoprotein syndrome, and postulate that it may allow for the detection of other carbohydrate\u2010deficient protein\u2010related disease states.<\/jats:p>","DOI":"10.1002\/elps.1150181017","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T11:37:48Z","timestamp":1117280268000},"page":"1819-1826","source":"Crossref","is-referenced-by-count":51,"title":["Capillary electrophoresis\u2010based separation of transferrin sialoforms in patients with carbohydrate\u2010deficient glycoprotein syndrome"],"prefix":"10.1002","volume":"18","author":[{"given":"Robert P.","family":"Oda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajani","family":"Prasad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert L.","family":"Stout","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Coffin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wendy P.","family":"Patton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel L.","family":"Kraft","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John F.","family":"O'Brien","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James P.","family":"Landers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,4,14]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.57.070188.004033"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00731704"},{"key":"e_1_2_1_4_2","first-page":"247","volume":"30","author":"Agamanolis D. 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