{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T21:55:01Z","timestamp":1762898101840,"version":"build-2065373602"},"reference-count":33,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2005,4,14]],"date-time":"2005-04-14T00:00:00Z","timestamp":1113436800000},"content-version":"vor","delay-in-days":3025,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[1997,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Pharmaceuticals in human plasma are determined on underivatized fused\u2010silica capillaries by micellar electrokinetic capillary chromatography (MEKC) without sample pretreatment. Our best method to date uses as running buffer a sodium dodecyl sulfate (SDS) containing borate buffer (60 m<jats:sc>M<\/jats:sc> with 200 m<jats:sc>M<\/jats:sc> SDS) at pH 10. Between runs, proteins adsorbed to the capillary wall are removed by an acetonitrile and SDS\u2010buffer rinsing regimen (50% v\/v each). A day\u2010to\u2010day precision for relative peak areas of about 2% relative standard deviation (RSD; <jats:italic>n<\/jats:italic> &gt;40) has been reached. Different rinsing approaches are discussed (salts, enzyme\u2010containing solutions, organic solvents, hydrofluoric acid). The separation system is tested in a concentration range between approximately 100 mg\/L\u201010 mg\/L. Correlations between the limit of quantitation, the limit of detection and the signal\/noise are discussed. The applicability of the system is demonstrated for the pharmaceuticals acetaminophen, salicylic acid, sulfamethoxazole, tolbutamide, and trimethoprim.<\/jats:p>","DOI":"10.1002\/elps.1150181026","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T11:37:48Z","timestamp":1117280268000},"page":"1882-1889","source":"Crossref","is-referenced-by-count":37,"title":["Determination of pharmaceuticals in plasma by capillary electrophoresis without sample pretreatment reproducibility, limit of quantitation and limit of detection"],"prefix":"10.1002","volume":"18","author":[{"given":"Annette","family":"Kunkel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"G\u00fcnter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hermann","family":"W\u00e4tzig","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,4,14]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(95)00247-K"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(95)00063-O"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1006\/mchj.1996.1421"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(92)80047-T"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(95)00622-4"},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","first-page":"1805","DOI":"10.1093\/clinchem\/42.11.1805","volume":"42","author":"Zhang C.\u2010X.","year":"1996","journal-title":"Clin. Chem."},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(95)01202-8"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.11501501197"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.2116\/analsci.12.177"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(95)00701-6"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(95)00364-S"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(95)00115-4"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150150103"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150150104"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/40.10.1904"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(95)00242-B"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9797(88)90001-X"},{"key":"e_1_2_1_19_2","unstructured":"W\u00e4tzig H. Habilitation Thesis University of W\u00fcrzburg1994 pp.184\u2013185."},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(96)00441-4"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007691-199308000-00009"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150160112"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(97)00068-X"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02290363"},{"volume-title":"Hagers Handbuch der Pharmazeutischen Praxis","year":"1993","author":"von Bruchhausen F.","key":"e_1_2_1_25_2"},{"volume-title":"DAB 10 (German Pharmacopoeia) and 1st\u20133rd Supplement","year":"1996","key":"e_1_2_1_26_2"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1351\/pac199264040455"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0169-7439(93)80009-7"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150150109"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(94)01047-I"},{"volume-title":"Analytical Profiles of Drug Substances","year":"1991","author":"Florey K.","key":"e_1_2_1_31_2"},{"key":"e_1_2_1_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0099-5428(08)60698-X"},{"key":"e_1_2_1_33_2","unstructured":"W\u00e4tzig H. Habilitation Thesis University of W\u00fcrzburg1994 p.189."},{"key":"e_1_2_1_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(92)80296-7"}],"container-title":["ELECTROPHORESIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Felps.1150181026","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.1150181026","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T12:51:27Z","timestamp":1760532687000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/elps.1150181026"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,1]]},"references-count":33,"journal-issue":{"issue":"10","published-print":{"date-parts":[[1997,1]]}},"alternative-id":["10.1002\/elps.1150181026"],"URL":"https:\/\/doi.org\/10.1002\/elps.1150181026","archive":["Portico"],"relation":{},"ISSN":["0173-0835","1522-2683"],"issn-type":[{"type":"print","value":"0173-0835"},{"type":"electronic","value":"1522-2683"}],"subject":[],"published":{"date-parts":[[1997,1]]}}}