{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T01:24:53Z","timestamp":1760577893412,"version":"build-2065373602"},"reference-count":23,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,4,14]],"date-time":"2005-04-14T00:00:00Z","timestamp":1113436800000},"content-version":"vor","delay-in-days":2601,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[1998,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Scrapie in sheep and goats is the prototype of transmissible spongiform encephalopathies found in humans and animals. A feature of these diseases is the accumulation of rod\u2010shaped fibrils in the brain that form from an aggregated protein. This protein (PrP<jats:sup>SC<\/jats:sup>) is a protease\u2010resistant form of a normal host cell protein. When the aggregated protein is denatured in sodium dodecyl sulfate (SDS) and \u03b2\u2010mercaptoethanol, a monomer form of \u223c27 kDa molecular mass is observed. A competition immunoassay to detect PrP<jats:sup>SC<\/jats:sup> from scrapie\u2010infected sheep was developed using free zone capillary electrophoresis with laser\u2010induced fluorescence (LIF) for detection and flourecein\u2010labeled sysnthetic peptides from PrP<jats:sup>SC<\/jats:sup>. Antibodies were made to each respective peptide and used in the competition assay. The fluorescent\u2010labeled peptides bound to the antibody were separated from the unbound peptides using 200 m<jats:sc>M<\/jats:sc> Tricine, pH 8.0, containing 0.1% <jats:italic>n<\/jats:italic>\u2010octylglucoside and 0.1% bovine serum albumin (BSA). The amount of antibody that would bind \u223c50% of the fluorescent\u2010labeled peptide was determined for each peptide. When unlabeled peptide was added to the assay, \u223c2 fmoles of the peptide could be measured. When PrP<jats:sup>SC<\/jats:sup> extracted from infected sheep brain was added to the assay, approximately 135 pg of PrP<jats:sup>SC<\/jats:sup> could be detected. When preparations from normal sheep were assayed, there was little or no competition for the bound peptides. Assays using two of the peptides, peptides spanning amino acid positions 142\u2013154 and 155\u2013178, clearly differentiated scrapie\u2010positive sheep from normal animals. This assay is a new method that can be used to diagnose scrapie and, possibly, other transmissible spongiform encephalopathies in animals and in humans.<\/jats:p>","DOI":"10.1002\/elps.1150190308","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T09:55:23Z","timestamp":1117274123000},"page":"409-414","source":"Crossref","is-referenced-by-count":44,"title":["A diagnostic test for scrapie\u2010infected sheep using a capillary electrophoresis immunoassay with fluorescent\u2010labeled peptides"],"prefix":"10.1002","volume":"19","author":[{"given":"Mary Jo","family":"Schmerr","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Allen","family":"Jenny","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,4,14]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-1128(199609)16:5<487::AID-MED4>3.0.CO;2-R"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.123.1.14"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.121.18.419"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(96)91412-9"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560021220"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(96)00610-2"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1177\/104063879300500301"},{"key":"e_1_2_1_9_2","doi-asserted-by":"crossref","first-page":"1228","DOI":"10.1128\/jcm.34.5.1228-1231.1996","volume":"34","author":"van Keulen L. 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