{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T15:10:54Z","timestamp":1770822654830,"version":"3.50.1"},"reference-count":36,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,4,14]],"date-time":"2005-04-14T00:00:00Z","timestamp":1113436800000},"content-version":"vor","delay-in-days":2540,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[1998,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cutaneous T cell lymphomas (CTCL) can be differentiated from benign inflammatory dermatoses by the demonstration of clonal T cells in skin biopsy. As a marker of the T cell clonality, the rearrangement of the T cell receptor (TCR) genes is amplified by polymerase chain reaction (PCR) and subsequently analyzed by several electrophoresis techniques. Since the validity of this approach depends substantially on the separating capacity of the applied electrophoresis technique, we investigated in the present study the lower detection limit and the sensitivity of heteroduplex\u2010loaded polyacrylamide gel electrophoresis on MDE\u00ae (mutation detection enhancement) gels (HD\u2010MDE PAGE), of heteroduplex\u2010loaded temperature gradient gel electrophoresis (HD\u2010TGGE) and fragment analysis (FA) on sequencing gels. Genomic DNA from formalin\u2010fixed, paraffin\u2010embedded skin biopsies of 53 CTCL specimens and 27 samples of benign dermatoses was analyzed by TCR<jats:sub>y<\/jats:sub> PCR followed by electrophoretic separation. Clonality was detected by HD\u2010MDE PAGE in 22, by HD\u2010TGGE in 34, and by FA in 33 of the 53 CTCL cases. Additionally, FA revealed an oligoclonal fragment profile in seven CTCL specimens. In the 27 samples from benign dermatoses, HD\u2010MDE PAGE and HD\u2010TGGE showed the expected polyclonal pattern in 26, and FA in 25 specimens. HD\u2010TGGE and FA detected a clonal pattern down to a dilution of 10<jats:sup>3<\/jats:sup> monoclonal cells in 10<jats:sup>6<\/jats:sup> peripheral blood mononuclear cells (PBMC), while HD\u2010MDE PAGE revealed a detection limit of 10<jats:sup>4<\/jats:sup> monoclonal cells in 10<jats:sup>6<\/jats:sup> PBMC. In conclusion, HD\u2010TGGE and FA possess a higher sensitivity and lower detection limit than HD\u2010MDE PAGE. Therefore, both former techniques are useful tools for the routine diagnostic procedure. With regard to time and cost, we recommend HD\u2010TGGE.<\/jats:p>","DOI":"10.1002\/elps.1150190507","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T12:48:58Z","timestamp":1117284538000},"page":"653-658","source":"Crossref","is-referenced-by-count":26,"title":["Detection of clonal T cells in cutaneous T cell lymphoma by polymerase chain reaction: Comparison of mutation detection enhancement\u2010polyacrylamide gel electrophoresis, temperature gradient gel electrophoresis and fragment analysis of sequencing gels"],"prefix":"10.1002","volume":"19","author":[{"given":"Uta","family":"Scheller","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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