{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T15:28:45Z","timestamp":1760887725238,"version":"build-2065373602"},"reference-count":12,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2005,4,14]],"date-time":"2005-04-14T00:00:00Z","timestamp":1113436800000},"content-version":"vor","delay-in-days":2356,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[1998,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>1\u2010Phenyl\u20103\u2010methyl\u20105\u2010pyrazolone (PMP) derivatives of component monosaccharides in glycoproteins (fucose, galactose, mannose, <jats:italic>N<\/jats:italic>\u2010acetylgalactosamine and <jats:italic>N<\/jats:italic>\u2010acetylglucosamine) and epimeric aldopentoses (arabinose, lyxose, ribose and xylose) were well separated from each other by capillary electrochromatography on a Hypersil ODS column with a mixture of 50 m<jats:sc>M<\/jats:sc> <jats:italic>N<\/jats:italic>\u2010(2\u2010hydroxyethyl)piperazine\u20102\u2032\u2010(2\u2010ethanesulfonic acid) buffer, pH 6.0 to \u223c 6.3, and acetonitrile (2.2:1 v\/v) as eluent. The elution of these compounds showed relatively strong dependence on the pH and concentration of the buffer salts contained in the eluent, as compared to the elution by pressure\u2010driven high\u2010performance liquid chromatography (HPLC) on the same stationary phase, but separation of PMP\u2010monosaccharides was better than that by HPLC. Retention times of PMP\u2010monosaccharides were highly reproducible with a relative standard deviation (RSD) of \u223c 0.6%, and quantification with an RSD less than 5% could be achieved using 3\u2010O\u2010methylglucose as an internal standard.<\/jats:p>","DOI":"10.1002\/elps.1150191518","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T12:36:50Z","timestamp":1117283810000},"page":"2682-2688","source":"Crossref","is-referenced-by-count":20,"title":["Separation of 1\u2010phenyl\u20103\u2010methyl\u20105\u2010pyrazolone derivatives of monosaccharides by capillary electrochromatography"],"prefix":"10.1002","volume":"19","author":[{"given":"Shigeo","family":"Suzuki","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mayumi","family":"Yamamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuri","family":"Kuwahara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyoko","family":"Makiura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susumu","family":"Honda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,4,14]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)90842-2"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)82057-9"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02321428"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac9717245"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1080\/10408349608050577"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(89)90444-2"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(92)80300-J"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(94)80046-4"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(96)00612-7"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(97)00803-0"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(95)01000-9"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/jhrc.1240180906"}],"container-title":["ELECTROPHORESIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Felps.1150191518","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.1150191518","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T17:55:41Z","timestamp":1760550941000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/elps.1150191518"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,11]]},"references-count":12,"journal-issue":{"issue":"15","published-print":{"date-parts":[[1998,11]]}},"alternative-id":["10.1002\/elps.1150191518"],"URL":"https:\/\/doi.org\/10.1002\/elps.1150191518","archive":["Portico"],"relation":{},"ISSN":["0173-0835","1522-2683"],"issn-type":[{"type":"print","value":"0173-0835"},{"type":"electronic","value":"1522-2683"}],"subject":[],"published":{"date-parts":[[1998,11]]}}}