{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T09:33:46Z","timestamp":1778924026193,"version":"3.51.4"},"reference-count":23,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2004,3,1]],"date-time":"2004-03-01T00:00:00Z","timestamp":1078099200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2004,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Alexa dye staining of proteins is used for the fluorescence microscopy of single particles that are sometimes multimolecular protein complexes. To characterize the staining, post\u2010staining determination must be made of which protein(s) in a complex have been Alexa\u2010stained. The present communication describes the use of sodium dodecyl sulfate\u2010polyacrylamide gel electrophoresis (SDS\u2010PAGE) for performing this determination. The Alexa\u2010stained proteins are observed directly in gels by illumination with an ultraviolet transilluminator. The test multimolecular particle is bacteriophage T7. The protein capsid of T7 is a multimolecular complex that has both external and internal proteins. SDS\u2010PAGE of Alexa\u2010stained bacteriophage T7 produces fluorescent capsid proteins each of which usually comigrates with an unstained protein. However, one Alexa\u2010induced modification of protein migration was observed by SDS\u2010PAGE. Mass spectrometry shows that the protein with modified migration is the major protein of the outer shell of the T7 capsid. The procedures used are generally applicable. The distribution of Alexa staining among T7 capsid proteins depends on the size of the dye molecule used. The larger the dye molecule is, the greater the preference for external proteins.<\/jats:p>","DOI":"10.1002\/elps.200305723","type":"journal-article","created":{"date-parts":[[2004,3,1]],"date-time":"2004-03-01T20:17:57Z","timestamp":1078172277000},"page":"779-784","source":"Crossref","is-referenced-by-count":12,"title":["Analysis of proteins stained by Alexa dyes"],"prefix":"10.1002","volume":"25","author":[{"given":"Shijun","family":"Huang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Houyi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher A.","family":"Carroll","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shirley J.","family":"Hayes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan T.","family":"Weintraub","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip","family":"Serwer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,3]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1064103"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1006\/jsbi.2001.4412"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(99)77011-6"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2443.1997.1450343.x"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1101\/SQB.1983.047.01.114"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1996.0251"},{"key":"e_1_2_1_8_2","first-page":"1","volume-title":"Electron Microscopy of Proteins","author":"Steven A. 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