{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T14:54:12Z","timestamp":1770130452087,"version":"3.49.0"},"reference-count":16,"publisher":"Wiley","issue":"10-11","license":[{"start":{"date-parts":[[2004,6,2]],"date-time":"2004-06-02T00:00:00Z","timestamp":1086134400000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2004,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The identification of diphenhydramine (DH) metabolites that are frequently observed in the capillary zone electrophoresis (CZE) and micellar electrokinetic capillary chromatography (MEKC) analyses of alkaline liquid\/liquid and solid\u2010phase extracts of patient urines is demonstrated. Having standards for DH and diphenhydramine\u2010<jats:italic>N<\/jats:italic>\u2010oxide (DHNO), the presence of these two compounds could be confirmed in urines that were collected overnight after administration of 25 mg DH chloride. Using CZE coupled to ion\u2010trap mass spectrometry (CE\u2010MS<jats:sup>n<\/jats:sup>) with positive electrospray ionization and an acetate buffer at pH 5.6, the [M+H]<jats:sup>+<\/jats:sup> ions of DH (<jats:italic>m\/z<\/jats:italic> = 256), DHNO (<jats:italic>m\/z<\/jats:italic> = 272), and nordiphenhydramine (NDH, <jats:italic>m\/z<\/jats:italic> = 242) and their fragmentation to a common <jats:italic>m\/z<\/jats:italic> 167 product ion (diphenylcarbinol moiety) was monitored. The data indicate that all three compounds are cations in an acidic environment, the migration order being NDH, DH, and DHNO. Data obtained under negative electrospray ionization conditions suggest the presence of diphenylmethoxyacetic acid\u2010glycine amide ([M\u2010H]<jats:sup>\u2212<\/jats:sup> ion of <jats:italic>m\/z<\/jats:italic> 298 and fragmentation to <jats:italic>m\/z<\/jats:italic> 254, loss of CO<jats:sub>2<\/jats:sub>), a metabolite that could tentatively be assigned to a characteristic peak observed in the MEKC electropherogram at alkaline pH. The data presented in this paper illustrate the value of using CE\u2010MS<jats:sup>n<\/jats:sup> for identification of urinary drug metabolites for which no standards are available.<\/jats:p>","DOI":"10.1002\/elps.200305829","type":"journal-article","created":{"date-parts":[[2004,6,2]],"date-time":"2004-06-02T05:01:37Z","timestamp":1086152497000},"page":"1607-1614","source":"Crossref","is-referenced-by-count":37,"title":["Identification of diphenhydramine metabolites in human urine by capillary electrophoresis\u2010ion trap\u2010mass spectrometry"],"prefix":"10.1002","volume":"25","author":[{"given":"Andrea","family":"Baldacci","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francine","family":"Prost","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolfgang","family":"Thormann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,6,2]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"264","volume-title":"Disposition of Toxic Drugs and Chemicals in Man","author":"Baselt R. 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