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Many studies have shown that reduced glutathione (GSH) and cysteine (Cys) deficiency play an important role in the infection. We have developed capillary zone electrophoresis (CZE)\u2010based assays and have used them to investigate the relationship between plasma and intracellular thiol levels and HIV\u20101 viremia in plasma. Blood samples from healthy volunteers and seropositive patients undergoing different antiretroviral regimes were analyzed in the study. The VL assay was based on CZE\u2010UV detection of viral RNA at 260\u00a0nm. Determination of endogenous reduced Cys and GSH was achieved by CZE\u2010UV detection of their mercurial complexes at 200\u00a0nm. We found that a decrease in GSH and Cys levels may be associated with disease progress. In fact, reduced GSH and Cys levels appear progressively reduced with increasing VL.<\/jats:p>","DOI":"10.1002\/elps.200305848","type":"journal-article","created":{"date-parts":[[2004,6,2]],"date-time":"2004-06-02T05:01:00Z","timestamp":1086152460000},"page":"1522-1529","source":"Crossref","is-referenced-by-count":36,"title":["Quantitation of reduced glutathione and cysteine in human immunodeficiency virus\u2010infected patients"],"prefix":"10.1002","volume":"25","author":[{"given":"Elena","family":"Sbrana","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriano","family":"Paladini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilia","family":"Bramanti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria C.","family":"Spinetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giorgio","family":"Raspi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,6,2]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(91)90297-B"},{"key":"e_1_2_1_3_2","doi-asserted-by":"crossref","first-page":"1921","DOI":"10.4049\/jimmunol.146.6.1921","volume":"146","author":"Smyth M. 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