{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T09:07:57Z","timestamp":1789808877556,"version":"4.0.1"},"reference-count":19,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2004,7,14]],"date-time":"2004-07-14T00:00:00Z","timestamp":1089763200000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2004,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have analyzed unfractionated sera of newly diagnosed patients (<jats:italic>n<\/jats:italic> = 10) with breast carcinoma (BC), prior to treatment, and patients (<jats:italic>n<\/jats:italic> = 5) with fibrocystic disease of the breast (FDB) by two\u2010dimensional gel electrophoresis (2\u2010DE) and silver staining. The patients' 2\u2010DE serum protein profiles obtained were then subjected to image analysis and compared to similar data generated from sera of normal healthy female controls (<jats:italic>n<\/jats:italic>\u00a0= 10) of the same range of age. The relative expression of \u03b1<jats:sub>1<\/jats:sub>\u2010antichymotrypsin (ACT),\u00a0clusterin, and complement factor B was significantly higher in all BC patients as compared to normal controls. However, the expression of \u03b1<jats:sub>1<\/jats:sub>\u2010antitrypsin (AAT) in BC patients was apparently lower than that of the controls. Similar differential expression of ACT was detected in the FDB patients. The aberrant expression of the serum acute\u2010phase proteins of patients with BC and FDB was confirmed by competitive enzyme\u2010linked immunosorbent assay (ELISA). Similar altered proteins expression was also observed from immunohistochemical studies of malignant (<jats:italic>n<\/jats:italic> = 5) and benign (<jats:italic>n<\/jats:italic> = 5) breast lesions of the respective patients performed using antisera to the aberrantly expressed proteins. However, the malignant breast lesions were instead positively stained for AAT. The differential expression of the serum proteins was apparently abrogated when a six\u2010month follow\u2010up study was performed on nine of the BC patients subsequent to treatment.<\/jats:p>","DOI":"10.1002\/elps.200305950","type":"journal-article","created":{"date-parts":[[2004,7,14]],"date-time":"2004-07-14T11:08:57Z","timestamp":1089803337000},"page":"2392-2401","source":"Crossref","is-referenced-by-count":45,"title":["Aberrant expression of acute\u2010phase reactant proteins in sera and breast lesions of patients with malignant and benign breast tumors"],"prefix":"10.1002","volume":"25","author":[{"given":"Saeid R.","family":"Doustjalali","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rohana","family":"Yusof","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng\u2010Har","family":"Yip","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lai\u2010Meng","family":"Looi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bhanumathy","family":"Pillay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Onn H.","family":"Hashim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,7,14]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199902113400607"},{"key":"e_1_2_1_3_2","first-page":"20","volume":"24","author":"van Leeuwen M. 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