{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T10:01:08Z","timestamp":1760954468667,"version":"build-2065373602"},"reference-count":27,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2003,2,24]],"date-time":"2003-02-24T00:00:00Z","timestamp":1046044800000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2003,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Rapid and highly reproducible nonreducing agarose gel electrophoresis (NRAGE) of von Willebrand Factor (vWF) multimers was performed using a thermostated minigel apparatus that monitors and precisely controls internal gel temperature. The substitution of lithium dodecyl sulfate (LiDS) for sodium dodecyl sulfate (SDS) allowed electrophoresis to be performed below the 16\u00b0C Krafft point of SDS and facilitated NRAGE of vWF over the entire range of 0\u201335\u00b0C. Internal gel temperature was regulated by a thermocouple probe inserted directly into the gel during electrophoresis which interfaced with a thermopilot that continually measures and adjusts temperature to within \u00b1 0.5\u00b0C. At 10\u00b0C operative temperature, NRAGE at 1.5% agarose concentration was completed in 20\u00a0min at 250\u00a0V. Electrophoresis could be performed in only 10\u00a0min at 500\u00a0V, but at such high voltages, localized temperature fluctuations as much as 6\u00b0C resulted in perturbation of banding patterns in those vicinities. In the optimized method, both high molecular weight multimers and proteolytic fragments of vWF were separable suggesting clinical applicability of this system for the diagnosis of von Willebrand Disease and thrombotic thrombocytopenic purpura.<\/jats:p>","DOI":"10.1002\/elps.200390068","type":"journal-article","created":{"date-parts":[[2003,2,24]],"date-time":"2003-02-24T18:00:47Z","timestamp":1046109647000},"page":"582-587","source":"Crossref","is-referenced-by-count":9,"title":["Rapid high\u2010resolution electrophoresis of multimeric von Willebrand Factor using a thermopiloted gel apparatus"],"prefix":"10.1002","volume":"24","author":[{"given":"Gary B.","family":"Smejkal","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John R.","family":"Shainoff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kandice M.","family":"Kottke\u2010Marchant","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,2,24]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00359a015"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00399a013"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V88.8.2939.bloodjournal8882939"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112129"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.67.1.395"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_1_8_2","first-page":"65","volume":"6","author":"Shainoff J. 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