{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:40:45Z","timestamp":1761057645819,"version":"build-2065373602"},"reference-count":8,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2003,3,4]],"date-time":"2003-03-04T00:00:00Z","timestamp":1046736000000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2003,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The emergence of a new environmentally caused variant of Creutzfeldt\u2010Jakob disease (vCJD), the result of food\u2010born infection by the causative agent of bovine spongiform encephalopathy (BSE), has stimulated research on a practical diagnostic screening test. The immunocompetitive capillary electrophoresis (ICCE) assay has been reported to detect disease\u2010specific, proteinase\u2010resistant prion protein (PrPres) in the blood of scrapie\u2010infected sheep. We have applied this method to blood from CJD\u2010infected chimpanzees and humans. The threshold of detection achieved with our ICCE was 0.6\u00a0n<jats:sc>M<\/jats:sc> of synthetic peptide corresponding to the prion protein (PrP) <jats:italic>C<\/jats:italic>\u2010terminus, and 2\u00a0n<jats:sc>M<\/jats:sc> of recombinant human PrP at the optimized conditions. However, the test was unable to distinguish between extracts of leucocytes from healthy and CJD\u2010infected chimpanzees, and from healthy human donors and patients affected with various forms of CJD. Thus, the ICCE assay as presently performed is not suitable for use as a screening test in human transmissible spongiform encephalopathies (TSEs).<\/jats:p>","DOI":"10.1002\/elps.200390107","type":"journal-article","created":{"date-parts":[[2003,3,18]],"date-time":"2003-03-18T11:11:17Z","timestamp":1047985877000},"page":"853-859","source":"Crossref","is-referenced-by-count":30,"title":["Failure of immunocompetitive capillary electrophoresis assay to detect disease\u2010specific prion protein in buffy coat from humans and chimpanzees with Creutzfeldt\u2010Jakob disease"],"prefix":"10.1002","volume":"24","author":[{"given":"Larisa","family":"Cervenakova","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Brown","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Serguei","family":"Soukharev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oksana","family":"Yakovleva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heino","family":"Diringer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evgueni L.","family":"Saenko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William N.","family":"Drohan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,3,4]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(96)91412-9"},{"key":"e_1_2_1_3_2","first-page":"839","volume-title":"Neurodegenerative Diseases","author":"Brown P.","year":"1994"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1067\/mlc.2001.111951"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-83-11-2897"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(99)00514-2"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150190308"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.15.5409"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410350504"}],"container-title":["ELECTROPHORESIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Felps.200390107","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.200390107","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T16:29:19Z","timestamp":1760545759000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/elps.200390107"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,3]]},"references-count":8,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2003,3]]}},"alternative-id":["10.1002\/elps.200390107"],"URL":"https:\/\/doi.org\/10.1002\/elps.200390107","archive":["Portico"],"relation":{},"ISSN":["0173-0835","1522-2683"],"issn-type":[{"type":"print","value":"0173-0835"},{"type":"electronic","value":"1522-2683"}],"subject":[],"published":{"date-parts":[[2003,3]]}}}