{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T10:28:03Z","timestamp":1791196083136,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2003,3,19]],"date-time":"2003-03-19T00:00:00Z","timestamp":1048032000000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2003,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Since biologically active peptides usually exhibit their effects in low concentrations, the development of sensitive analytical methods has become a challenge. In this paper, a multidimensional system is presented, consisting of a size\u2010exclusion chromatographic (SEC) separation followed by a trapping procedure on a 4\u00a0mm\u00d73\u00a0mm ID reversed\u2010phase C18 (RP18) column with subsequent elution of the trapped fraction and separation by capillary zone electrophoresis (CZE). The system has been tested with mixtures of six enkephalins and albumin, mimicking biological matrices such as plasma and cerebrospinal fluid. After separation of albumin from the enkephalins in the SEC dimension a heart\u2010cut of 200\u00a0\u03bcL, containing the enkephalin peak, is taken, concentrated on the RP18 microcolumn and, after elution with a 20\u00a0\u03bcL plug of 80% acetonitrile, electrokinetically injected into the CZE system, where stacking and separation is achieved. While validation shows generally good linearity and reproducibility, the quantitation limit with UV detection is acceptable (2.5\u00a0\u03bcg\/ mL with an injection volume of 50\u00a0\u03bcL).<\/jats:p>","DOI":"10.1002\/elps.200390132","type":"journal-article","created":{"date-parts":[[2003,3,20]],"date-time":"2003-03-20T10:58:47Z","timestamp":1048157927000},"page":"1126-1134","source":"Crossref","is-referenced-by-count":27,"title":["On\u2010line coupling of size\u2010exclusion chromatography and capillary zone electrophoresis <b><i>via<\/i><\/b> a reversed\u2010phase C18 trapping column for the determination of peptides in biological samples"],"prefix":"10.1002","volume":"24","author":[{"given":"Thom","family":"Stroink","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pim","family":"Schravendijk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerard","family":"Wiese","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Teeuwsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henk","family":"Lingeman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joop C. 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