{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:58:49Z","timestamp":1791291529372,"version":"4.3.0"},"reference-count":27,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2005,6,27]],"date-time":"2005-06-27T00:00:00Z","timestamp":1119830400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2005,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We describe the use of capillary eletrophoresis (CE) coupled with mass spectrometry (MS) to identify single polypeptides and patterns of polypeptides specific for prostate cancer (CaP) in human urine. Using improved sample preparation methods that enable enhanced comparability between different samples, we examined samples from 47\u2009patients who underwent prostate biopsy. Of this group, 21\u2009patients had benign pathology and 26 with\u2005CaP, and these were used to define potential biomarkers, which allow discrimination between these two states. In addition, CE\u2010MS data from these 47\u2009urine samples were compared to that of 41\u2009young men (control) without known or suspected clinical CaP to further confirm the polypeptides indicative for CaP. Upon crossvalidation of the same samples, several polypeptides were selected that enabled correct classification of the CaP patients with 92% sensitivity and 96% specificity. We then examined an additional 474\u2005samples from patients with renal disease enrolled in other studies and found that 14 (3%) had polypeptides suggestive of CaP possibly indicating that they harbor clinical CaP. In conclusion, this early pilot study suggests that CE\u2010MS of urine warrants further investigation as a tool that can identify putative biomarkers for CaP.<\/jats:p>","DOI":"10.1002\/elps.200400208","type":"journal-article","created":{"date-parts":[[2005,6,27]],"date-time":"2005-06-27T05:47:24Z","timestamp":1119851244000},"page":"2797-2808","source":"Crossref","is-referenced-by-count":132,"title":["Pilot study of capillary electrophoresis coupled to mass spectrometry as a tool to define potential prostate cancer biomarkers in urine"],"prefix":"10.1002","volume":"26","author":[{"given":"Dan","family":"Theodorescu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danilo","family":"Fliser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Wittke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harald","family":"Mischak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald","family":"Krebs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Walden","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Ross","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elke","family":"Eltze","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olaf","family":"Bettendorf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Wulfing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Axel","family":"Semjonow","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,7,29]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300661"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2003.07.013"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200400918"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.med.55.091902.105237"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btg484"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djh056"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1294"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200305788"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1755.2004.00659.x"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1755.2005.00335.x"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)12713-4"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00240-002-0238-5"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000139993.51181.5d"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-02-0518"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1009715923555"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1042\/CS20040103"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200305788"},{"key":"e_1_2_1_19_2","author":"Kolch W.","year":"2005","journal-title":"Mass Spectrom. 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