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A 5\u00a0m<jats:sc>M<\/jats:sc> sodium phosphate buffer, pH 7.40, was chosen as the most suitable CE buffer and an extensive ultrafiltration (UF) procedure was applied to purify the LDL sample. Two LDL particle species, LDL with lower mobility and LDL<jats:sup>\u2212<\/jats:sup> with higher mobility were observed. The electropherograms were highly reproducible with good precision of effective mobilities, corrected peak areas (CPAs) and CPA ratio of LDL<jats:sup>\u2212<\/jats:sup>\/LDL. LDL particles shown on the electropherogram were also characterized by several procedures. The applications of Sigma HDL cholesterol reagent and CE on\u2010line 2\u2010propanol precipitation indicated that the two particle species shown in the electropherogram belong to LDL. The LDL particles were found to associate with the buoyant LDL fraction and the LDL<jats:sup>\u2212<\/jats:sup> particles associate with the dense LDL fraction. This study utilizes CZE for the profiling of LDL isoforms and provides a new analytical method for the resolution of LDL subspecies. It demonstrates a high\u2010mobility LDL particle which circulates in healthy subjects and diminishes in atherosclerotic patients. Diminution of the high\u2010mobility LDL subspecies may be linked to minimal formation of arterial plaque in atherosclerotic patients.<\/jats:p>","DOI":"10.1002\/elps.200406055","type":"journal-article","created":{"date-parts":[[2004,8,30]],"date-time":"2004-08-30T06:02:51Z","timestamp":1093845771000},"page":"2985-2995","source":"Crossref","is-referenced-by-count":5,"title":["Charge density profiling of circulating human low\u2010density lipoprotein particles by capillary zone electrophoresis"],"prefix":"10.1002","volume":"25","author":[{"given":"Mine\u2010Yine","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine J.","family":"McNeal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronald D.","family":"Macfarlane","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,8,30]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.11.2.298"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9150(92)90255-F"},{"key":"e_1_2_1_4_2","first-page":"1.24","volume-title":"Cholesterol and Atherosclerosis: Diagnosis and Treatment","author":"Grundy S. 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