{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T19:41:41Z","timestamp":1760557301666,"version":"build-2065373602"},"reference-count":24,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2005,6,2]],"date-time":"2005-06-02T00:00:00Z","timestamp":1117670400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2005,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Short tandem repeat (STR) loci, widely used as genetic markers in disease diagnostic studies and human identity applications, are traditionally genotyped through comparison of allele sizes to a sequenced allelic ladder. Allelic ladders permit a floating bin allele calling method to be utilized, which enables reliable allele calling across laboratories, instrument platforms, and electrophoretic conditions. Precise sizing methods for STR allele calling involving fixed bins can also be used when a high degree of precision has been demonstrated within an instrument platform and a set of electrophoretic conditions. An alternative method for reliable genotyping of STR markers, locus\u2010specific brackets (LSBs), is introduced here. LSBs are artificial alleles created through molecular biology manipulations to be shorter or longer than alleles commonly seen in populations under investigation. The size and repeat number of measured alleles are interpolated between the two LSB products that are mixed with the polymerase chain reaction\u2010amplified STR alleles. The advantages and limitations of the LSB approach are described along with a concordance study between the LSB typing approach and other STR typing methods. Complete agreement was observed with 162\u2009samples studied at 5\u2005Y\u2010chromosome loci.<\/jats:p>","DOI":"10.1002\/elps.200410404","type":"journal-article","created":{"date-parts":[[2005,6,3]],"date-time":"2005-06-03T11:49:27Z","timestamp":1117799367000},"page":"2583-2590","source":"Crossref","is-referenced-by-count":1,"title":["Locus\u2010specific brackets for reliable typing of Y\u2010chromosome short tandem repeat markers"],"prefix":"10.1002","volume":"26","author":[{"given":"John M.","family":"Butler","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jill E.","family":"Appleby","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David L.","family":"Duewer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,7,4]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"53","volume-title":"Forensic DNA Typing: Biology and Technology behind STR Markers","author":"Butler J. M.","year":"2001"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1520\/JFS15445J"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1520\/JFS2002195"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0379-0738(02)00195-0"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150190116"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.11501601163"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004140050197"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00201a010"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1520\/JFS14227J"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.19.3925"},{"volume-title":"Sizing and quantitation of polymerase chain reaction products by capillary electrophoresis for use in DNA typing","year":"1995","author":"Butler J.M.","key":"e_1_2_1_12_2"},{"key":"e_1_2_1_13_2","unstructured":"Dau P.C. Liu D.DNA bracketing locus compatible standards for electrophoresis U.S. Patent 6 013 444."},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004140050167"},{"key":"e_1_2_1_15_2","first-page":"953","volume":"53","author":"Puers C.","year":"1993","journal-title":"Am. J. Hum. Genet."},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.19.10273"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1999.4067"},{"key":"e_1_2_1_18_2","volume":"5","author":"Klein S.B.","year":"2003","journal-title":"Forensic Science Communications"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200305822"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1520\/JFS2003045"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1520\/JFS13521J"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1520\/JFS2003043"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0379-0738(00)00412-6"},{"key":"e_1_2_1_24_2","first-page":"91","volume":"15","author":"Butler J.M.","year":"2003","journal-title":"Forensic Sci. Rev."},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.forsciint.2003.10.007"}],"container-title":["ELECTROPHORESIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Felps.200410404","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.200410404","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T18:59:13Z","timestamp":1760554753000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/elps.200410404"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,7]]},"references-count":24,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2005,7]]}},"alternative-id":["10.1002\/elps.200410404"],"URL":"https:\/\/doi.org\/10.1002\/elps.200410404","archive":["Portico"],"relation":{},"ISSN":["0173-0835","1522-2683"],"issn-type":[{"type":"print","value":"0173-0835"},{"type":"electronic","value":"1522-2683"}],"subject":[],"published":{"date-parts":[[2005,7]]}}}