{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T01:31:31Z","timestamp":1792114291797,"version":"4.4.0"},"reference-count":54,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2005,11,15]],"date-time":"2005-11-15T00:00:00Z","timestamp":1132012800000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2005,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Haplotypic variation within and among the <jats:italic>Ascaris<\/jats:italic> populations representing six provinces in China was investigated. Mitochondrial DNA regions in the cytochrome <jats:italic>c<\/jats:italic> oxidase subunit 1 (<jats:italic>cox<\/jats:italic>1) and NADH dehydrogenase subunit 1 (<jats:italic>nad<\/jats:italic>1) genes were amplified by PCR from total genomic DNA samples (<jats:italic>n<\/jats:italic>\u2005&gt;\u2005720) from <jats:italic>Ascaris<\/jats:italic> individuals from humans and pigs, and subjected to mutation scanning and subsequent selective sequencing. For the <jats:italic>cox<\/jats:italic>1, ten different electrophoretic profiles were recorded for human <jats:italic>Ascaris<\/jats:italic>, and the same number for pig <jats:italic>Ascaris<\/jats:italic>, one of them being common to both host species. For the <jats:italic>nad<\/jats:italic>1, 11 different profiles were detected for human <jats:italic>Ascaris<\/jats:italic>, and 15 for pig <jats:italic>Ascaris<\/jats:italic>. Having defined all haplotypes (20 for p<jats:italic>cox<\/jats:italic>1 and 26 for p<jats:italic>nad<\/jats:italic>1) by sequencing, their frequencies were estimated in each of the two host species and each of the six provinces. For each mitochondrial region, the frequency of the different haplotypes varied considerably, depending on host species and geographical origin. Analysis of the sequence data (representing all haplotypes for each mitochondrial locus) by <jats:italic>F<\/jats:italic>\u2010statistics indicated restricted gene flow between human <jats:italic>Ascaris<\/jats:italic> and pig <jats:italic>Ascaris<\/jats:italic>, and supported the conclusions from previous molecular epidemiological investigations that pigs are not a significant source of <jats:italic>Ascaris<\/jats:italic> infection in humans in endemic regions.<\/jats:p>","DOI":"10.1002\/elps.200500276","type":"journal-article","created":{"date-parts":[[2005,11,16]],"date-time":"2005-11-16T19:21:34Z","timestamp":1132168894000},"page":"4317-4326","source":"Crossref","is-referenced-by-count":75,"title":["Mutation scanning\u2010coupled analysis of haplotypic variability in mitochondrial DNA regions reveals low gene flow between human and porcine <b><i>Ascaris<\/i><\/b> in endemic regions of China"],"prefix":"10.1002","volume":"26","author":[{"given":"Weidong","family":"Peng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keng","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianmin","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin B.","family":"Gasser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,11,15]]},"reference":[{"key":"e_1_2_1_2_2","volume-title":"Report of the WHO Informal Consultation on the Use of Chemotherapy for the Control of Morbidity due to Soil\u2010Transmitted Nematodes in Humans","year":"1996"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(08)60423-6"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000006442"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/0-306-47383-6_3"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.2527\/jas1988.6661548x"},{"key":"e_1_2_1_7_2","first-page":"253","volume-title":"Ascariasis and Its Prevention and Control","author":"Anderson R. 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