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After careful study of the derivatization condition such as pH value, reagent concentration, temperature, and reaction time, derivatization reaction was accomplished as quickly as 10\u2005min with stable yield. Optimal separation of SAMF\u2010labeled amines was achieved with a running buffer (pH\u20059.3) containing 30\u2005mM boric acid, 25\u2005mM SDS, and 20%\u2005v\/v ACN. The proposed method allowed biogenic amines to be determined with LODs as low as 0.25\u20132.5\u2005nmol\/L and RSD values from 0.4 to 4.5%. The present method has been successfully used to monitor biogenic amines in HeLa cells and fish samples. This study exploits the potential of MEKC\u2010LIF with SAMF labeling as a tool for monitoring biogenic amines involved in complex physiological and behavioral processes in various matrices.<\/jats:p>","DOI":"10.1002\/elps.200500301","type":"journal-article","created":{"date-parts":[[2006,1,13]],"date-time":"2006-01-13T04:47:41Z","timestamp":1137127661000},"page":"827-836","source":"Crossref","is-referenced-by-count":21,"title":["Determination of biogenic amines in HeLa cell lysate by 6\u2010oxy\u2010(<b><i>N<\/i><\/b>\u2010succinimidyl acetate)\u20109\u2010(2'\u2013methoxycarbonyl) fluorescein and micellar electrokinetic capillary chromatography with laser\u2010induced fluorescence detection"],"prefix":"10.1002","volume":"27","author":[{"given":"Liwei","family":"Cao","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huashan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,2,8]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(01)00395-4"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2362.2000.00585.x"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00054a009"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(97)00011-5"},{"key":"e_1_2_1_6_2","first-page":"759","volume":"48","author":"Pegg A. 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