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We succeeded in establishing a new MCL cell line, called MAVER\u20101, from a leukemic MCL, and performed a thorough phenotypical, cytogenetical and molecular characterization of the cell line. In the present report, the phenotypic expression of GRANTA\u2010519 and MAVER\u20101 cell lines has been compared and evaluated by a proteomic approach, exploiting 2\u2010D map analysis. By univariate statistical analysis (Student's <jats:italic>t<\/jats:italic>\u2010test, as commonly used in most commercial software packages), most of the protein spots were found to be identical between the two cell lines. Thirty spots were found to be unique for the GRANTA\u2010519, whereas another 11\u2009polypeptides appeared to be expressed only by the MAVER\u20101 cell line. A number of these spots could be identified by MS. These data were confirmed and expanded by multivariate statistical tools (principal component analysis and soft\u2010independent model of class analogy) that allowed identification of a larger number of differently expressed spots. Multivariate statistical tools have the advantage of reducing the risk of false positives and of identifying spots that are significantly altered in terms of correlated expression rather than absolute expression values. It is thus suggested that, in future work in differential proteomic profiling, both univariate and multivariate statistical tools should be adopted.<\/jats:p>","DOI":"10.1002\/elps.200500323","type":"journal-article","created":{"date-parts":[[2005,12,21]],"date-time":"2005-12-21T12:17:21Z","timestamp":1135167441000},"page":"484-494","source":"Crossref","is-referenced-by-count":26,"title":["Multivariate statistical tools applied to the characterization of the proteomic profiles of two human lymphoma cell lines by two\u2010dimensional gel electrophoresis"],"prefix":"10.1002","volume":"27","author":[{"given":"Emilio","family":"Marengo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisa","family":"Robotti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"Bobba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Cristina","family":"Liparota","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiara","family":"Rustichelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto","family":"Zam\u00f2","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"Chilosi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pier Giorgio","family":"Righetti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,1,25]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/0471709158"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/mas.10075"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200305457"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200390020"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200305950"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/mc.10159"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300726"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300712"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200305683"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200305574"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300550"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300797"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300799"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300822"},{"key":"e_1_2_1_16_2","volume-title":"Chemometrics: A Textbook","author":"Massart D. 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