{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:53:39Z","timestamp":1761058419421,"version":"build-2065373602"},"reference-count":36,"publisher":"Wiley","issue":"5-6","license":[{"start":{"date-parts":[[2006,3,7]],"date-time":"2006-03-07T00:00:00Z","timestamp":1141689600000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2006,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Helicobacter pylori<\/jats:italic> is a major human pathogen that is responsible for a number of gastrointestinal infections. We have used 2\u2010DE to characterise protein synthesis in bacteria grown either on solid agar\u2010based media or in each of two broth culture media (Brucella and brain heart infusion (BHI) broth). Significant differences were observed in the proteomes of bacteria grown either on agar\u2010based or in broth media. Major changes in protein abundance were identified using principal component analysis (PCA), which delineated the profiles derived for the three key growth conditions (<jats:italic>i.e.<\/jats:italic> agar plates, Brucella and BHI broth). Proteins detected across the gel series were identified by peptide mass mapping and Edman sequencing. A number of proteins associated with protein synthesis in general as well as specific amino acid synthesis were depressed in broth\u2010grown bacteria compared to plate\u2010grown bacteria. A similar reduction was also observed in the abundance of proteins involved in detoxification. Two of the most abundant spots, identified as UreB and GroEL, in plate\u2010grown bacteria showed a &gt;140\u2010fold drop in abundance in bacteria grown in Brucella broth compared to bacteria grown on agar plates. Two protein spots induced in bacteria grown in broth culture were both identified as glyceraldehyde 3\u2010phosphate dehydrogenase based on their <jats:italic>N<\/jats:italic>\u2010terminal amino acid sequences derived by Edman degradation. The underlying causes of the changes in the proteins abundance were not clear, but it was likely that a significant proportion of the changes were due to the alkaline pH of the broth culture media.<\/jats:p>","DOI":"10.1002\/elps.200500655","type":"journal-article","created":{"date-parts":[[2006,3,7]],"date-time":"2006-03-07T17:23:29Z","timestamp":1141752209000},"page":"1136-1146","source":"Crossref","is-referenced-by-count":19,"title":["Growth\u2010induced changes in the proteome of <b><i>Helicobacter pylori<\/i><\/b>"],"prefix":"10.1002","volume":"27","author":[{"given":"Christina","family":"Uwins","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Deitrich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evelyn","family":"Argo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth","family":"Stewart","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian","family":"Davidson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Phil","family":"Cash","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,3,7]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(03)00024-6"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(99)00652-3"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1026051530512"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.6.3033"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2002.tb11287.x"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.5.2643-2655.2003"},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"4501","DOI":"10.1128\/iai.64.11.4501-4507.1996","volume":"64","author":"Karita M.","year":"1996","journal-title":"Infect. 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