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In order to identify proteins that undergo modifications as a result of synaptic activation, we induced massive exocytosis and analysed the synaptic vesicle compartment by benzyldimethyl\u2010<jats:italic>n<\/jats:italic>\u2010hexadecylammonium chloride (BAC)\/SDS\u2010PAGE and difference gel electrophoresis (DIGE) followed by MALDI\u2010TOF\u2010MS. We identified eight proteins that revealed significant changes in abundance following nerve terminal depolarization. Of these, six were increased and two were decreased in abundance. Three of these proteins were phosphorylated as detected by Western blot analysis. In addition, we identified an unknown synaptic vesicle protein whose abundance increased on synaptic activation. Our results demonstrate that depolarization of the presynaptic compartment induces changes in the abundance of synaptic vesicle proteins and post\u2010translational protein modification.<\/jats:p>","DOI":"10.1002\/elps.200500864","type":"journal-article","created":{"date-parts":[[2006,8,30]],"date-time":"2006-08-30T12:06:42Z","timestamp":1156939602000},"page":"3488-3496","source":"Crossref","is-referenced-by-count":35,"title":["Synaptic vesicle proteins under conditions of rest and activation: Analysis by 2\u2010D difference gel electrophoresis"],"prefix":"10.1002","volume":"27","author":[{"given":"Jacqueline","family":"Burr\u00e9","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Beckhaus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carsten","family":"Corvey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Karas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herbert","family":"Zimmermann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walter","family":"Volknandt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,8,30]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1294.018"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.neuro.26.041002.131412"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300817"},{"key":"e_1_2_1_5_2","first-page":"1732","volume":"95","author":"Morciano M.","year":"2005","journal-title":"J. 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