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Binding of BS<jats:sup>3<\/jats:sup> to proteins was apparent in electrophoresis. In three proteins of 3T3\u2010L1 cells, protein kinase\u2010C\u03f5, protein kinase\u2010C\u03b6, and glyceraldehyde\u20103\u2010phosphate dehydrogenase, BS<jats:sup>3<\/jats:sup> action was detectable in SDS\u2010PAGE with immunoblotting. This enabled confirmation of the well\u2010known intracellular localization of these proteins. In cathepsin E of erythrocytes, a mobility increase in nondenaturing PAGE was the most prominent effect of BS<jats:sup>3<\/jats:sup> treatment. A mechanism for the increase in mobility due to BS<jats:sup>3<\/jats:sup> binding is suggested. 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