{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:45:56Z","timestamp":1760575556848,"version":"build-2065373602"},"reference-count":22,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2006,10,23]],"date-time":"2006-10-23T00:00:00Z","timestamp":1161561600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Entamoeba histolytica<\/jats:italic> is a pathogenic protozoan parasite, which causes amoebic colitis, dysentery and liver abscesses in humans. Since the cyst and small trophozoite stages of this parasite are indistinguishable by light microscopy from <jats:italic>Entamoeba dispar<\/jats:italic> (which is nonpathogenic), specific diagnosis is compromised. To overcome this limitation, a PCR\u2010coupled SSCP approach, utilising a sequence difference of 4.6% in a short region (\u223c173\u2013174\u2005bp) of the small subunit of nuclear ribosomal DNA, was evaluated for the differentiation of the two species of <jats:italic>Entamoeba<\/jats:italic>. Including a range of well\u2010defined control DNA samples (<jats:italic>n<\/jats:italic>\u00a0=\u00a067) to evaluate the specificity of the PCR, 45\u2005DNA samples representing <jats:italic>E.\u2005histolytica<\/jats:italic> and <jats:italic>E.\u2005dispar<\/jats:italic> from human faecal samples were tested by SSCP, and unequivocal delineation between the species was achieved. This SSCP approach should provide a practical tool for the specific diagnosis of <jats:italic>E.\u2005histolytica<\/jats:italic> in humans and for investigating its epidemiology.<\/jats:p>","DOI":"10.1002\/elps.200600211","type":"journal-article","created":{"date-parts":[[2006,10,23]],"date-time":"2006-10-23T08:01:45Z","timestamp":1161590505000},"page":"4419-4422","source":"Crossref","is-referenced-by-count":0,"title":["Differentiation of <b><i>Entamoeba histolytica<\/i><\/b> from <b><i>Entamoeba dispar<\/i><\/b> by PCR\u2010coupled nonisotopic SSCP analysis"],"prefix":"10.1002","volume":"27","author":[{"given":"Jaco J.","family":"Verweij","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bronwyn E.","family":"Campbell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robin B.","family":"Gasser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,11,21]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"93","volume-title":"Amebiais: Human Infection by Entamoeba histolytica","author":"Walsh A. 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