{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:23:51Z","timestamp":1762100631032,"version":"build-2065373602"},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2007,2,16]],"date-time":"2007-02-16T00:00:00Z","timestamp":1171584000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2007,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The composition of single MCF\u20107 breast cancer cells is characterized using 2\u2010D CE. Individual MCF\u20107 cells were aspirated into a 30\u2005\u03bcm inner diameter fused\u2010silica capillary and lysed by contact with an SDS\u2010containing buffer. Proteins and other primary amines were fluorescently labeled on\u2010column using the fluorogenic dye 3\u2010(2\u2010furoyl)quinoline\u20102\u2010carboxaldehyde. Labeled components were separated first according to molecular weight using capillary sieving electrophoresis (CSE) and then by MEKC. Analytes were detected in a sheath\u2010flow cuvette using LIF. The expression profiles for MCF\u20107 cellular homogenate and a single MCF\u20107 cell are compared. As a proof\u2010of\u2010principle investigation, variation in expression was also compared within and between G1 and G2\/M cell cycle phases for MCF\u20107 cells. Following their treatment with the viable nuclear stain Hoechst 33342, MCF\u20107 cells were sorted by flow cytometry on the basis of their ploidy. Sorted cells were then analyzed by 2\u2010D CE. The degree of variability was &gt;2.5 times larger between cells of different phases than between cells of the same phase. In typical 1\u2005h 2\u2010D CE separations using MCF\u20107 cells, over 100 components are resolved.<\/jats:p>","DOI":"10.1002\/elps.200600500","type":"journal-article","created":{"date-parts":[[2007,2,16]],"date-time":"2007-02-16T09:58:06Z","timestamp":1171619886000},"page":"932-937","source":"Crossref","is-referenced-by-count":24,"title":["Cell cycle\u2010dependent characterization of single MCF\u20107 breast cancer cells by 2\u2010D CE"],"prefix":"10.1002","volume":"28","author":[{"given":"Melissa M.","family":"Harwood","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joan V.","family":"Bleecker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter S.","family":"Rabinovitch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norman J.","family":"Dovichi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,3,16]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.3322\/canjclin.47.1.28"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac034153r"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.14.7081"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0320(19990901)37:1<14::AID-CYTO2>3.0.CO;2-J"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-003-2253-2"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.10617"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(02)00071-6"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(98)00920-0"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac0498314"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac0156621"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2675314"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00046a031"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00169a027"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0270(94)90009-4"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00097a010"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.2001.5148"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac010939i"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/1522-2683(200109)22:17<3677::AID-ELPS3677>3.0.CO;2-Q"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac034154j"},{"volume-title":"Unified Separation Science","year":"1991","author":"Giddings J. C.","key":"e_1_2_1_21_2"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2003.08.031"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac050231u"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac000534i"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac0497499"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1039\/b206438c"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200405939"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.T100009-MCP200"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac960549"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2004.04.038"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)00528-4"},{"key":"e_1_2_1_32_2","doi-asserted-by":"crossref","first-page":"5977","DOI":"10.1021\/ac061029+","volume":"78","author":"Kraly J. R.","journal-title":"Anal. Chem."}],"container-title":["ELECTROPHORESIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Felps.200600500","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.200600500","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T14:11:00Z","timestamp":1760537460000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/elps.200600500"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,3]]},"references-count":31,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2007,3]]}},"alternative-id":["10.1002\/elps.200600500"],"URL":"https:\/\/doi.org\/10.1002\/elps.200600500","archive":["Portico"],"relation":{},"ISSN":["0173-0835","1522-2683"],"issn-type":[{"type":"print","value":"0173-0835"},{"type":"electronic","value":"1522-2683"}],"subject":[],"published":{"date-parts":[[2007,3]]}}}