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Both discoidal and spherical particles formed a monolayer on the inner silica wall as confirmed by atomic force microscopy. The effect of coating conditions, such as HDL concentration and coating time, was investigated with spherical HDL particles. Examination of the influence of pH on the coating stability also allowed the determination of p<jats:italic>I<\/jats:italic> values for the HDL particles attached to the capillary wall. The p<jats:italic>I<\/jats:italic> values for spherical and discoidal HDL particles were close to 5.0. The repeatabilities of the EOF mobility and the retention factors of the uncharged steroid hormones used as model compounds were exploited in the evaluation of the coating stability. The optimal coating was achieved with 0.1\u2005mg\/mL HDL protein and 50\u2005min flushing with coating solution followed by 15\u2005min standing time. Electrochromatography with HDL\u2010coated open tubular capillaries offers a new tool for the study of HDL particle structure and transformations.<\/jats:p>","DOI":"10.1002\/elps.200600766","type":"journal-article","created":{"date-parts":[[2007,7,2]],"date-time":"2007-07-02T08:30:50Z","timestamp":1183365050000},"page":"2267-2274","source":"Crossref","is-referenced-by-count":18,"title":["Coating of open tubular capillaries with discoidal and spherical high\u2010density lipoprotein particles in electrochromatography"],"prefix":"10.1002","volume":"28","author":[{"given":"Kati","family":"Vainikka","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jari","family":"Metso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matti","family":"Jauhiainen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marja\u2010Liisa","family":"Riekkola","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,7,2]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00041433-199412000-00003"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr020466v"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9150(01)00651-7"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-200363180-00003"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9150(99)00150-1"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)39898-9"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(95)00236-7"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi002815q"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1050-1738(00)00078-5"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000057572.97137.DD"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/1522-2683(200210)23:20<3615::AID-ELPS3615>3.0.CO;2-J"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150181015"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac011096y"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac052006i"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200600370"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI103182"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112247200"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)62718-3"},{"key":"e_1_2_1_20_2","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1016\/S0022-2275(20)32144-1","volume":"40","author":"Jauhiainen M.","year":"1999","journal-title":"J. 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