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In this study, we demonstrate the effectiveness of nonisotopic SSCP analysis of a \u223c300\u2005bp region of the small subunit (pSSU) of ribosomal DNA for the specific identification of and delineation among 18 different <jats:italic>Cryptosporidium<\/jats:italic> species and genotypes from a wide range of hosts. This mutation scanning approach allowed the rapid and reliable differentiation between species\/genotypes differing by as little as 1.3% in the pSSU sequence, with the capacity to detect point mutations. The present findings confirm the usefulness of this tool for the rapid genetic screening of <jats:italic>Cryptosporidium<\/jats:italic> samples from any host species, providing a foundation for detailed systematic, epidemiological and ecological studies. Although applied herein to pSSU, this low cost approach should be applicable to a wide range of genetic loci for population genetic investigations of <jats:italic>Cryptosporidium<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/elps.200600772","type":"journal-article","created":{"date-parts":[[2007,8,14]],"date-time":"2007-08-14T07:16:55Z","timestamp":1187075815000},"page":"2818-2825","source":"Crossref","is-referenced-by-count":28,"title":["Specific and genotypic identification of <b><i>Cryptosporidium<\/i><\/b> from a broad range of host species by nonisotopic SSCP analysis of nuclear ribosomal DNA"],"prefix":"10.1002","volume":"28","author":[{"given":"Aaron R.","family":"Jex","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Una M.","family":"Ryan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josephine","family":"Ng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bronwyn E.","family":"Campbell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lihua","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melita","family":"Stevens","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robin B.","family":"Gasser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,8,14]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(94)E0059-V"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.17.1.72-97.2004"},{"key":"e_1_2_1_4_2","doi-asserted-by":"crossref","first-page":"3984","DOI":"10.1128\/JCM.38.11.3984-3990.2000","volume":"38","author":"McLauchlin J.","year":"2000","journal-title":"J. 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