{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T15:14:13Z","timestamp":1766157253762,"version":"build-2065373602"},"reference-count":51,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2007,8,3]],"date-time":"2007-08-03T00:00:00Z","timestamp":1186099200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A highly sensitive in\u2010column fiber\u2010optic LIF detector for CE has been constructed and evaluated. In this detection system, a 457\u2010nm diode\u2010pumped solid\u2010state blue laser was used as the excitation light source and an optical fiber (40\u2005\u03bcm od) was used to transmit the excitation light. One end of the optical fiber was inserted into the separation capillary and was <jats:italic>in situ<\/jats:italic> positioned at the detection window. The other end of the fiber was protruded from the capillary to capture the excitation light beam from the blue laser. Fluorescence emission was collected by a 40\u00d7microscope objective, focused on a spatial filter, and passed through a yellow color filter before reaching the photomultiplier tube. The present CE\u2010fluorescence detection is a simple and compact optical system. It reduces the laser scattering effect from the capillary and fiber as compared to the conventional LIF detection for CE. Its utility was successfully demonstrated by the separation and determination of <jats:sc>D<\/jats:sc>\u2010penicillamine labeled with naphthalene\u20102,3\u2010dicarboxaldehyde. The detection limit was 0.8\u2005nM (S\/N\u2005=\u20053). The present detection scheme has been proven to be attractive for sensitive fluorescence detection for CE.<\/jats:p>","DOI":"10.1002\/elps.200600815","type":"journal-article","created":{"date-parts":[[2007,8,3]],"date-time":"2007-08-03T05:19:42Z","timestamp":1186118382000},"page":"3105-3114","source":"Crossref","is-referenced-by-count":21,"title":["In\u2010column fiber\u2010optic laser\u2010induced fluorescence detection for CE"],"prefix":"10.1002","volume":"28","author":[{"given":"Xiupei","family":"Yang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongyan","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunlin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shulin","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin M. 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