{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:37:26Z","timestamp":1760575046380,"version":"build-2065373602"},"reference-count":22,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2007,11,6]],"date-time":"2007-11-06T00:00:00Z","timestamp":1194307200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2007,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Neurodegenerative diseases such as Alzheimer disease (AD) and Parkinson disease (PD) have been associated with increased production of reactive oxygen species. In AD and PD patients, superoxide dismutase (SOD1) was also indicated as a major target of oxidative damage. In particular, in brain tissue of these patients, different SOD1 isoforms have been identified, although their functional role still remains to be elucidated. In the light of the possibility that different SOD1 entities could be expressed also in other neurodegenerative disorders, as a sort of unifying event with AD and PD, we have investigated amyotrophic lateral sclerosis (ALS) using human neuroblastoma SH\u2010SY5Y cells with mutated <jats:italic>SOD1<\/jats:italic> gene H46R as cellular model. 2\u2010DE using a narrow\u2010range IPG 4\u20137 strips in the first dimension and linear 15% SDS\u2010PAGE in the second allowed to separate different SOD1 spots. MALDI\u2010TOF MS and CapLC\u2010MS\/MS have been used for their complete identification. This is the first report in which the presence of SOD1 (iso)forms in a cellular model of ALS has been evidenced.<\/jats:p>","DOI":"10.1002\/elps.200700162","type":"journal-article","created":{"date-parts":[[2007,11,6]],"date-time":"2007-11-06T11:48:08Z","timestamp":1194349688000},"page":"4340-4347","source":"Crossref","is-referenced-by-count":2,"title":["Optimizing separation efficiency of 2\u2010DE procedures for visualization of different superoxide dismutase forms in a cellular model of amyotrophic lateral sclerosis"],"prefix":"10.1002","volume":"28","author":[{"given":"Cristina","family":"Di Poto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paolo","family":"Iadarola","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberta","family":"Salvini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ileana","family":"Passadore","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Cereda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mauro","family":"Ceroni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna Maria","family":"Bardoni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,11,26]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M414327200"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(03)00606-8"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1067"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(02)00780-3"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2006.01.057"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1997.69052064.x"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(01)02926-2"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M314124200"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1087753"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0730423100"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8351519"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1293-323"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(94)00885-X"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(97)01051-X"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.275.5303.1129"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200401031"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200305844"},{"key":"e_1_2_1_19_2","first-page":"1701","volume":"165","author":"Strey C. W.","year":"2004","journal-title":"Neurobiology"},{"key":"e_1_2_1_20_2","first-page":"281","volume":"61","author":"Cheon M. S.","year":"2001","journal-title":"J. Neural Transm. Suppl."},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/1522-2683(20000701)21:13<2610::AID-ELPS2610>3.0.CO;2-H"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/1522-2683(200108)22:14<2865::AID-ELPS2865>3.0.CO;2-Y"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00552a005"}],"container-title":["ELECTROPHORESIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Felps.200700162","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.200700162","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T11:03:49Z","timestamp":1760526229000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/elps.200700162"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,11,26]]},"references-count":22,"journal-issue":{"issue":"23","published-print":{"date-parts":[[2007,12]]}},"alternative-id":["10.1002\/elps.200700162"],"URL":"https:\/\/doi.org\/10.1002\/elps.200700162","archive":["Portico"],"relation":{},"ISSN":["0173-0835","1522-2683"],"issn-type":[{"type":"print","value":"0173-0835"},{"type":"electronic","value":"1522-2683"}],"subject":[],"published":{"date-parts":[[2007,11,26]]}}}