{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T17:29:54Z","timestamp":1789925394012,"version":"4.0.1"},"reference-count":22,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2007,7,31]],"date-time":"2007-07-31T00:00:00Z","timestamp":1185840000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We report an ultrasensitive method for the analysis of glycosphingolipid catabolism. The substrate G<jats:sub>M1<\/jats:sub> and the set of seven metabolites into which it can be degraded (G<jats:sub>A1<\/jats:sub>, G<jats:sub>M2<\/jats:sub>, G<jats:sub>A2<\/jats:sub>, G<jats:sub>M3<\/jats:sub>, LacCer, GlcCer, and Cer) were labeled with the highly fluorescent dye tetramethylrhodamine. CE with LIF detection was used to assay these compounds with 150\u2005\u00b1\u200580\u2005yoctomole mass (1\u2005ymol\u2005=\u200510<jats:sup>\u221224<\/jats:sup>\u2005mol\u2005=\u20050.6\u2005copies) detection limits and 5\u2005\u00b1\u20053\u2005pM concentration detection limits. An alignment algorithm based on migration of two components was employed to correct for drift in the separation. The within\u2010day and between\u2010day precision in peak height was 20%, in peak width 15%, and in adjusted migration time 0.03%. After normalization to total sample injected, the RSD in peak height reduced to 2\u20136%, which approaches the limit set by molecular shot noise in the number of molecules taken for analysis. PC12 cells were incubated with the labeled G<jats:sub>M1<\/jats:sub>. Fluorescent microscopy demonstrated uptake by the cells. CE was used to separate a cellular homogenate prepared from these cells. A set of peaks was observed, which were tentatively identified based on comigration with the standards. Roughly 120\u2005pL of homogenate was injected, which contained a total of 150\u2005zmol of labeled substrate and products. Metabolite that preserves the fluorescent label can be detected at the yoctomole level, which should allow characterization of this metabolic pathway in single cells.<\/jats:p>","DOI":"10.1002\/elps.200700202","type":"journal-article","created":{"date-parts":[[2007,7,31]],"date-time":"2007-07-31T12:16:27Z","timestamp":1185884187000},"page":"3100-3104","source":"Crossref","is-referenced-by-count":34,"title":["Yoctomole analysis of ganglioside metabolism in PC12 cellular homogenates"],"prefix":"10.1002","volume":"28","author":[{"given":"Colin D.","family":"Whitmore","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulf","family":"Olsson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. Andreas","family":"Larsson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ole","family":"Hindsgaul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica M.","family":"Palcic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norman J.","family":"Dovichi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,8,27]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R200001200"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2006.05.027"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R200010200"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1023\/B:GLYC.0000033627.02765.cc"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200307087"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresrev.2004.07.014"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.13.7532"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.276.5311.428"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0320(19990901)37:1<14::AID-CYTO2>3.0.CO;2-J"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2003.08.012"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3140381"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(01)84284-9"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/4.2.239"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2004.12.001"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00412a034"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.carres.2006.10.002"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac060704c"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/1522-2683(200209)23:17<3071::AID-ELPS3071>3.0.CO;2-K"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00155a023"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(99)00893-6"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac950651r"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac070716d"}],"container-title":["ELECTROPHORESIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Felps.200700202","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.200700202","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T10:10:13Z","timestamp":1760523013000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/elps.200700202"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,8,27]]},"references-count":22,"journal-issue":{"issue":"17","published-print":{"date-parts":[[2007,9]]}},"alternative-id":["10.1002\/elps.200700202"],"URL":"https:\/\/doi.org\/10.1002\/elps.200700202","archive":["Portico"],"relation":{},"ISSN":["0173-0835","1522-2683"],"issn-type":[{"value":"0173-0835","type":"print"},{"value":"1522-2683","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,8,27]]}}}