{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T10:39:07Z","timestamp":1791196747373,"version":"4.1.0"},"reference-count":57,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2007,10,26]],"date-time":"2007-10-26T00:00:00Z","timestamp":1193356800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2007,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In the present study, we used a mutation scanning\u2010targeted sequencing approach to assess variation in part (p<jats:italic>gp60<\/jats:italic>) of the 60 kDa glycoprotein (<jats:italic>gp60<\/jats:italic>) gene among <jats:italic>Cryptosporidium<\/jats:italic> samples from humans in Victoria, Australia. Two nuclear ribosomal loci (the small subunit rRNA gene and the second internal transcribed spacer) were used to identify the samples as <jats:italic>Cryptosporidium hominis<\/jats:italic> (<jats:italic>n<\/jats:italic>\u2005=\u200574), <jats:italic>Cryptosporidium parvum<\/jats:italic> (<jats:italic>n<\/jats:italic>\u2005=\u200523) or <jats:italic>Cryptosporidium meleagridis<\/jats:italic> (<jats:italic>n<\/jats:italic>\u2005=\u20051). In total, nine distinct p<jats:italic>gp60<\/jats:italic> sequences were identified (three <jats:italic>C. hominis<\/jats:italic>, five <jats:italic>C. parvum<\/jats:italic> and one <jats:italic>C. meleagridis<\/jats:italic>). Phylogenetic analyses of the p<jats:italic>gp60<\/jats:italic> sequence data, employing well\u2010defined reference sequences for comparison, allowed the genotypic and subgenotypic classification of samples. The <jats:italic>C. hominis<\/jats:italic> samples were classified as Ib A10G2R2, Id A15G1R2, and a new genotype, designated Ib2, was identified subgenotypically as A18G1R4. The <jats:italic>C. parvum<\/jats:italic> samples were classified as IIa A18G3R1, IIa A20G3R1, IIa A22G3R1, IIa A23G3R1 and IIc A5G3R2. These findings suggested that the <jats:italic>C. hominis<\/jats:italic> metapopulation is largely homogeneous, consisting of a single dominant genotype, Ib A10G2R2, whereas the <jats:italic>C. parvum<\/jats:italic> metapopulation is considerably more heterogeneous, with no single dominant genotype. The greater level of genetic heterogeneity found among the <jats:italic>C. parvum<\/jats:italic> samples, despite the smaller sample size, may relate to the zoonotic infection pattern of this species, which would be reflective of a greater number of possible infection sources. The present mutation scanning approach, coupled with targeted sequencing of genetically distinct representatives, is a practical, cost\u2010effective tool for large\u2010scale population genetic and epidemiological studies of <jats:italic>Cryptosporidium<\/jats:italic> and other eukaryotic organisms.<jats:fn><jats:p>Nucleotide sequence data reported in this paper are available in GenBank accession nos. 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