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The pCEC separation of urinary samples was performed on a RP column packed with C<jats:sub>18<\/jats:sub>, 5\u2005\u03bcm particles with an ACN\/water mobile phase containing TFA. The effects of the acid modifiers, applied voltage, mobile phase, and detection wavelength were systematically evaluated using eight spiked standards, as well as urine samples. A typical analytical trial of urine samples from Sprague Dawley (S.D.) rats exposed to high\u2010energy diet was carried out following sample pretreatment. Significant differences in urinary metabolic profiles were observed between the high energy diet\u2010induced obesity rats and the healthy control rats at the 6th\u2005wk postdose. Multivariate statistical analysis revealed the differential metabolites in response to the diet, which were partially validated with the putative standards. This work suggests that such a pCEC\u2010based separation and analysis method may provide a new and cost\u2010effective platform for metabolomic study uniquely positioned between the conventional chromatographic tools such as HPLC, and hyphenated analytical techniques such as LC\u2010MS.<\/jats:p>","DOI":"10.1002\/elps.200700420","type":"journal-article","created":{"date-parts":[[2007,11,2]],"date-time":"2007-11-02T13:30:13Z","timestamp":1194010213000},"page":"4459-4468","source":"Crossref","is-referenced-by-count":14,"title":["Analysis of urinary metabolites for metabolomic study by pressurized CEC"],"prefix":"10.1002","volume":"28","author":[{"given":"Guoxiang","family":"Xie","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingming","family":"Su","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue","family":"Gu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Yan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunping","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Houkai","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Jia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,11,26]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/cfg.82"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/004982599238047"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0958-1669(03)00023-5"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1202-802"},{"key":"e_1_2_1_6_2","first-page":"265","volume":"6","author":"Lindon J. 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