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The use of protein precipitation (PP) prior to a hydrodynamic injection (HD) was well adapted to high\u2010concentration samples (&gt;1\u2005ppm) and allowed high throughput. In contrast, the combination of liquid\u2013liquid extraction (LLE) and electrokinetic injection (EK) was time\u2010consuming but did allow detection at the ppb level. Both approaches were fully validated according to ICH guidelines and SFSTP protocols for two pharmaceutical compounds (ecstasy and methadone (MTD)). Deuterated internal standards (IS) in the analytical procedures were used and good quantitative performance was obtained in terms of trueness and precision (repeatability and intermediate precision) since accuracy profiles were within the acceptance limits (30% for biological assay). Methods were linear over the concentration range of 0.50\u2013175\u2005ng\/mL and 0.25\u20135\u2005\u03bcg\/mL for LLE\u2010EK and PP\u2010HD procedures, respectively. The LLE\u2010EK methodology was finally successfully applied to quantitation of ecstasy and MTD in real cases obtained from toxicology.<\/jats:p>","DOI":"10.1002\/elps.200700464","type":"journal-article","created":{"date-parts":[[2008,4,11]],"date-time":"2008-04-11T10:16:47Z","timestamp":1207909007000},"page":"2193-2202","source":"Crossref","is-referenced-by-count":48,"title":["Validation of chiral capillary electrophoresis\u2010electrospray ionization\u2010mass spectrometry methods for ecstasy and methadone in plasma"],"prefix":"10.1002","volume":"29","author":[{"given":"Julie","family":"Schappler","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Davy","family":"Guillarme","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josiane","family":"Prat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean\u2010Luc","family":"Veuthey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Serge","family":"Rudaz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,5,21]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(99)01309-6"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.11501501113"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-9936(98)00007-7"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-9936(98)00008-9"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac9700686"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(99)00417-X"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200290028"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200305791"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2004.10.010"},{"volume-title":"Guidance for Industry, Bioanalytical Method Validation","year":"2001","key":"e_1_2_1_11_2"},{"key":"e_1_2_1_12_2","first-page":"E43","volume":"9","author":"Shah V. 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