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When a concentrated yeast extract was analyzed by CE\u2010MS using a successive multiple ionic\u2010polymer layer (SMIL)\u2010coated capillary, the adsorption of the contaminants on the capillary wall caused severe problems such as no elution, band\u2010broadening, and asymmetric peaks. Therefore, an analytical method for the analysis of anionic metabolites in yeast was developed by pressure\u2010assisted CE using an inert polymer capillary made from poly(ether etherketone) (PEEK) and PTFE. We preferred to use the PEEK over the PTFE capillary in CE\u2010MS due to the easy\u2010to\u2010use PEEK capillary and its high durability. The separation of anionic metabolites was successfully achieved with ammonium hydrogencarbonate\/formate buffer (pH\u20056.0) as the electrolyte solution. The use of 2\u2010propanol washing after every electrophoresis run not only eliminated wall\u2010adsorption phenomena, but allowed for good repeatability to be obtained for migration times in the metabolomic analysis.<\/jats:p>","DOI":"10.1002\/elps.200700466","type":"journal-article","created":{"date-parts":[[2008,4,18]],"date-time":"2008-04-18T09:53:13Z","timestamp":1208512393000},"page":"2016-2023","source":"Crossref","is-referenced-by-count":27,"title":["Development of a capillary electrophoresis\u2010mass spectrometry method using polymer capillaries for metabolomic analysis of 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