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The poly(methyl methacrylate) (PMMA) multichannel chip was fabricated by CO<jats:sub>2<\/jats:sub> laser ablation and bonded with a fused\u2010silica separation capillary for CE\/FA separation with UV detection. The chip was designed to allow a complete assay of four electrophoretic runs using preconditioned channels to speed up the determination of free bilirubin and to deliver quick results for bedside monitoring. Under optimized conditions, the linear working range for free bilirubin was from 10 to 200\u2005\u03bcmol with RSDs from 2.1 to 5.0% for <jats:italic>n<\/jats:italic>\u2005=\u20053, and the LOD at 9\u2005\u03bcmol for S\/N\u2005=\u20053. From a binding study between bilirubin and HSA under FA condition, the second binding constant for bilirubin\u2013HSA was determined as 1.07\u00d710<jats:sup>5<\/jats:sup>\u2005L\/mol and the number of binding sites <jats:italic>per<\/jats:italic> HSA as 3.46. The results enabled the calculation of free bilirubin for jaundiced infants based on the clinically significant level of total bilirubin, producing a range of 118.3\u2013119.4\u2005\u03bcmol\/L. The developed method is shown to meet the clinical requirement with additional margin of protection to detect the early rising level of free bilirubin prior to jaundice condition. The low\u2010cost microchip CE\/FA device is shown to produce quick results with high potential to deliver a suitable bed\u2010side monitoring method for bilirubin management in neonates.<\/jats:p>","DOI":"10.1002\/elps.200700596","type":"journal-article","created":{"date-parts":[[2008,4,7]],"date-time":"2008-04-07T10:42:24Z","timestamp":1207564944000},"page":"1924-1931","source":"Crossref","is-referenced-by-count":46,"title":["Microchip capillary electrophoresis for frontal analysis of free bilirubin and study of its interaction with human serum albumin"],"prefix":"10.1002","volume":"29","author":[{"given":"Zhou","family":"Nie","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying Sing","family":"Fung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,4,28]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1536-4801.2004.tb00783.x"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0095-5108(18)30097-6"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.2000.4485"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0306-9877(95)90213-9"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.113.4.775"},{"key":"e_1_2_1_7_2","first-page":"448","volume":"110","author":"Yeung C. 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