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The adsorbed cat\u2010HEC coating exhibited minimal interactions with basic proteins, providing efficient basic protein separations with excellent reproducibility. Under broad pHs, the amine groups are the main charged groups bringing about a global positive charge on the capillary wall. As a consequence, the cat\u2010HEC coating produced an anodal EOF performance. A comparative study on the use of hydroxyethylcellulose (HEC) and cat\u2010HEC as physically adsorbed coatings for CE are also presented. The separation efficiency and analysis reproducibility proved that the cat\u2010HEC polymer was efficient in suppressing the adsorption of basic proteins onto the silica capillary wall. The long\u2010term stability of the cat\u2010HEC coating in consecutive protein separation runs has demonstrated the suitability of the coating for high\u2010throughput electrophoretic protein separations.<\/jats:p>","DOI":"10.1002\/elps.200700715","type":"journal-article","created":{"date-parts":[[2008,4,2]],"date-time":"2008-04-02T10:47:56Z","timestamp":1207133276000},"page":"1460-1466","source":"Crossref","is-referenced-by-count":32,"title":["Cationized hydroxyethylcellulose as a novel, adsorbed coating for basic protein separation by capillary electrophoresis"],"prefix":"10.1002","volume":"29","author":[{"given":"Runmiao","family":"Yang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronghua","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuhua","family":"Peng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanmei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,4,2]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac0012492"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac980522l"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200390029"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2683(19991001)20:15\/16<3106::AID-ELPS3106>3.0.CO;2-2"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2683(20000501)21:8<1521::AID-ELPS1521>3.0.CO;2-Y"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00214a009"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(98)00851-6"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac980231c"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac970041m"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/1522-2683(200109)22:17<3813::AID-ELPS3813>3.0.CO;2-D"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200390150"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200400091"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac970999h"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150180120"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00085a019"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)00090-X"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150190215"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2683(20000301)21:5<909::AID-ELPS909>3.0.CO;2-L"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac960260s"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(94)85085-2"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(96)00226-9"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(95)00394-3"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(95)00960-4"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac960432l"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(96)00385-4"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(03)01175-0"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200500692"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200500429"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(03)00739-8"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)00665-8"},{"key":"e_1_2_1_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2005.06.097"},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1039\/b316492f"},{"key":"e_1_2_1_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200500124"},{"key":"e_1_2_1_35_2","unstructured":"Hiroyuki N. 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