{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T19:07:12Z","timestamp":1790968032234,"version":"4.1.0"},"reference-count":26,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2008,5,16]],"date-time":"2008-05-16T00:00:00Z","timestamp":1210896000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2008,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>ACE technique provides an effective tool for the separation and identification of disease\u2010related biomarkers in clinical analysis. In recent years, a couple of synthetic DNA or RNA oligonucleotides, known as aptamers, rival the specificity and affinity for targets to antibodies and are employed as one kind of powerful affinity probe in ACE. In this work, based on high affinity between antithrombin aptamer and thrombin (their dissociation constant is 0.5\u2005nM), a carboxyfluorescein\u2010labeled 29\u2010nucleotide (nt) aptamer (F29\u2010mer) was used and an aptamer\u2010based affinity probe CE (aptamer\u2010based APCE) method was successfully established for high\u2010sensitive detection and quantitative analysis of thrombin. Experimental conditions including incubation temperature and time, buffer composition, and concentration of cations were investigated and optimized. Under the optimized condition, the linear range was from 0 to 400\u2005nM and the LOD was 2\u2005nM (74\u2005ng\/mL, S\/N\u2005=\u20053), <jats:italic>i.e.<\/jats:italic>, 40\u2005amol, both in running buffer and in 5% v\/v human serum. This LOD is the lowest one than those achieved by the previous APCE methods but based on a 15\u2010mer aptamer. This approach offers a promising method for the rapid, selective, and sensitive detection of thrombin in practical utility. Further binding experiments using one carboxyfluorescein\u2010labeled aptamer and the other nonlabeled aptamer or <jats:italic>vice versa<\/jats:italic> were carried out to deduce the formation of ternary complex when these two aptamers coexisted in the free solution with thrombin.<\/jats:p>","DOI":"10.1002\/elps.200700798","type":"journal-article","created":{"date-parts":[[2008,5,16]],"date-time":"2008-05-16T07:46:23Z","timestamp":1210923983000},"page":"2570-2577","source":"Crossref","is-referenced-by-count":51,"title":["High\u2010sensitive determination of human \u03b1\u2010thrombin by its 29\u2010mer aptamer in affinity probe capillary electrophoresis"],"prefix":"10.1002","volume":"29","author":[{"given":"Yilin","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fucheng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoyang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jijun","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianwei","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,6,20]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2005.06.031"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac049842x"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac053344m"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2004.11.006"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/346818a0"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac0519864"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0831159100"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0601755103"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac980638h"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac060021x"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(00)02131-1"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/355564a0"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1997.1275"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200390176"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac049158i"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-04-1710"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110257200"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.2000.4848"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja0603958"},{"key":"e_1_2_1_21_2","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1007\/978-1-4615-7658-7","volume-title":"Principles of Fluorescence Spectroscopy","author":"Lakowicz J. 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